Genetic and Molecular Studies on Short Integument

短珠被的遗传和分子研究

• 批准号: 9728239
• 负责人: Animesh Ray
• 金额: $ 20万
• 依托单位: University of Rochester
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-02-01 至 2000-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9728239&HistoricalAwards=false
• 关键词: Genetic; Molecular; Studies; Short; Integument

Ray 9728239 The gene SHORT INTEGUMENT (SIN1) is an important regulator of reproductive and vegetative morphogenesis in Arabidopsis thaliana. Mutations in this gene cause uncoordinated morphogenesis of ovule integument cells, cause a heterochronic shift in the program of meristem fate change, and, so far uniquely in plants, produce a pronounced maternal-effect disruption of embryonic pattern formation. These preliminary studies indicate that SIN1 plays an important role in cell fate specification. We have now proposed two models that address the mechanism by which SIN1 controls meristem fate. Here we propose to: I. Clone the SIN 1 gene and determine its sequence. II. Test genetic models of how SIN1 regulates flowering time by continuing our analysis of the effects of sin1 mutation on functions of the meristem identity genes APETALA2, and LEAFY, as far as possible during this funding period. Commitment to inflorescence development is the first important step towards reproductive development in flowering plants. The last important step constitutes fertilization and embryo development which occur within the ovules. The preliminary work described here has established a genetic connection between the earliest and the last steps in plant reproduction. The studies proposed here investigate the mechanisms behind this connection.

射线 9728239 SIN 1基因是拟南芥生殖和营养形态建成的重要调控因子。该基因的突变导致胚珠珠被细胞的形态发生不协调，导致分生组织命运变化程序的异时性转变，并且迄今为止在植物中是唯一的，产生明显的母体效应破坏胚胎模式形成。这些初步研究表明，SIN 1在细胞命运的规范中起着重要作用。我们现在已经提出了两个模型，解决机制，SIN 1控制分生组织的命运。在此，我们建议： I. 克隆SIN 1基因并测定其序列. 二. 在此资助期间，尽可能继续分析sin 1突变对分生组织身份基因APETALA 2和LEAFY功能的影响，以测试sin 1如何调节开花时间的遗传模型。 花序发育是开花植物生殖发育的第一个重要步骤。最后一个重要步骤是受精和胚胎发育，发生在胚珠内。这里描述的初步工作已经建立了植物繁殖最早和最后步骤之间的遗传联系。这里提出的研究调查了这种联系背后的机制。