Genetic and Molecular Studies on Short Integument

基因SHORT INTEGUMENT （SIN1）是拟南芥（Arabidopsis thaliana）生殖和营养形态发生的重要调控因子。该基因的突变导致胚珠被细胞的形态发生不协调，导致分生组织命运变化程序的异时性改变，并且，迄今为止在植物中唯一的，产生明显的母体效应破坏胚胎模式形成。这些初步研究表明，SIN1在细胞命运规范中起着重要作用。我们现在提出了两个模型来解释SIN1控制分生组织命运的机制。在此，我们建议：1 .克隆sin1基因并确定其序列。2。通过继续分析SIN1突变对分生组织识别基因APETALA2和LEAFY功能的影响，在本研究资助期内尽可能地测试SIN1如何调节开花时间的遗传模型。对花序发育的承诺是开花植物生殖发育的第一步。最后一个重要步骤是受精和胚胎发育，发生在胚珠内。这里描述的初步工作已经在植物繁殖的最早和最后步骤之间建立了遗传联系。本文提出的研究探讨了这种联系背后的机制。

Ray 9728239 The gene SHORT INTEGUMENT (SIN1) is an important regulator of reproductive and vegetative morphogenesis in Arabidopsis thaliana. Mutations in this gene cause uncoordinated morphogenesis of ovule integument cells, cause a heterochronic shift in the program of meristem fate change, and, so far uniquely in plants, produce a pronounced maternal-effect disruption of embryonic pattern formation. These preliminary studies indicate that SIN1 plays an important role in cell fate specification. We have now proposed two models that address the mechanism by which SIN1 controls meristem fate. Here we propose to: I. Clone the SIN 1 gene and determine its sequence. II. Test genetic models of how SIN1 regulates flowering time by continuing our analysis of the effects of sin1 mutation on functions of the meristem identity genes APETALA2, and LEAFY, as far as possible during this funding period. Commitment to inflorescence development is the first important step towards reproductive development in flowering plants. The last important step constitutes fertilization and embryo development which occur within the ovules. The preliminary work described here has established a genetic connection between the earliest and the last steps in plant reproduction. The studies proposed here investigate the mechanisms behind this connection.