Homologous Recombination in Arabidopsis Induced by HO Endonuclease

H2O 核酸内切酶诱导拟南芥中的同源重组

• 批准号: 9630402
• 负责人: Animesh Ray
• 金额: $ 20万
• 依托单位: University of Rochester
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-08-01 至 1998-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9630402&HistoricalAwards=false
• 关键词: Homologous; Recombination; Arabidopsis; Induced; HO

Ray 9630402 The integrity, as well as the diversity of chromosomes, is maintained by recombination and yet little is known about how this mechanism is judiciously performed, particularly in plants. A system of induced mitotic recombination in Arabidopsis thaliana, in which a single, defined, DNA double strand break is delivered to the chromosome at a particular locus has been developed. The target site of a site-specific DNA endonuclease, HO endonuclease of yeast, is cloned into a unique chromosomal location and the break induced by the expression of a cloned HO gene in the plant. Specific objectives are 1) to test the hypothesis that a chromosomal double strand break in meiosis can induce homologous recombination in Arabidopsis and 2) to determine whether ectopic recombination can be stimulated in mitosis and meiosis by a DNA double strand break in the plant chromosome. %%% A better understanding of the basic mechanisms involved in recombination of plant chromosomes will result from this study using the model plant, Arabidopsis thalania. Moreover, any movement toward the goal of achieving site-specific recombination in plants will have significant impact in both basic and applied research.

射线 9630402染色体的完整性和多样性是通过重组来维持的，但关于这种机制是如何明智地进行的，特别是在植物中，人们知之甚少。在拟南芥中诱导有丝分裂重组的系统，其中一个单一的，确定的，DNA双链断裂被传递到染色体上的一个特定的基因座已经开发。将位点特异性DNA核酸内切酶（酵母的HO核酸内切酶）的靶位点克隆到独特的染色体位置，并且通过克隆的HO基因在植物中的表达诱导断裂。具体目标是1）检验减数分裂中染色体双链断裂可以诱导拟南芥中同源重组的假设和2）确定植物染色体中DNA双链断裂是否可以在有丝分裂和减数分裂中刺激异位重组。 本研究以拟南芥为模式植物，对植物染色体重组的基本机制有了更好的理解。此外，任何朝着实现植物位点特异性重组目标的进展都将对基础研究和应用研究产生重大影响。