U.S.-Australia Cooperative Research: Algal Virus RestrictionModification Enzymes

美澳合作研究：藻类病毒限制修饰酶

• 批准号: 9215816
• 负责人: James Van Etten
• 金额: $ 0.77万
• 依托单位: University of Nebraska-Lincoln
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-05-01 至 1996-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9215816&HistoricalAwards=false
• 关键词: U.S.; Australia; Cooperative; Research; Algal

This award supports the travel of Dr. James Van Etten of the University of Nebraska at Lincoln to Australia to conduct collaborative research with Dr. Jan Rohozinski of the Research School of Biological Sciences at the Australian National University. The goal of the research is to understand the biological function of DNA methyltransferases and DNA restriction enzymes coded by viruses which infect eukaryotic alga. Algal viruses are icosahedral DNA containing viruses which infect chlorella cells. Specifically, the research will attempt to establish a transformation system in chlorella so that studies of algal viral genes can be undertaken. Work to develop the transformation system is to be carried out in Australia at the Australian National University in Canberra. Once the transformation system is established, the cloning of viral genes will be accomplished. The facilities at the University of Nebraska are recognized as a major center for studying these viruses and the restriction modification system they encode. The analysis of the restriction modification genes, isolated by the Australian investigators, will be done at the University of Nebraska.

该奖项资助内布拉斯加大学林肯分校的James Van Etten博士前往澳大利亚，与澳大利亚国立大学生物科学研究院的Jan Rohozinski博士进行合作研究。这项研究的目的是了解感染真核藻的病毒编码的DNA甲基转移酶和DNA限制性内切酶的生物学功能。藻类病毒是一种含有二十面体DNA的病毒，可以感染小球藻细胞。具体地说，这项研究将试图建立小球藻的转化系统，以便进行藻类病毒基因的研究。开发改造系统的工作将在澳大利亚堪培拉的澳大利亚国立大学进行。一旦转化系统建立，病毒基因的克隆就完成了。内布拉斯加大学的设施被认为是研究这些病毒及其编码的限制性内切酶修饰系统的主要中心。由澳大利亚研究人员分离出来的限制性内切酶修饰基因的分析将在内布拉斯加大学进行。