Purchase of a Fluorescence-Activated Cell Sorter

购买荧光激活细胞分选仪

• 批准号: 9217403
• 负责人: Robert Murphy
• 金额: $ 14.09万
• 依托单位: Carnegie-Mellon University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-02-15 至 1995-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9217403&HistoricalAwards=false
• 关键词: Purchase; Fluorescence; Activated; Cell; Sorter

This proposal is for the purchase of a fluorescence- activated cell sorter to replace one purchased in 1983 at the inception of the Center for Fluorescence Research in Biomedical Sciences at Carnegie Mellon University. The PI and co-PI are leaders in the flow cytometry community and the current FACS has been used in 38 research publications in cell biology in the 9 years since its installation. No other instrument exists at CMU. Limitations in its capabilities compared to newer instruments and difficulties with repairing and maintaining it justify replacement. The group of major and minor users receives over $3,290,000 in direct cost research support per year from federal and other agencies, including over $690,000 per year from NSF. The proposal replacement instrument will be used for research in a number of areas of cell, molecular and developmental biology, including membrane traffic, somatic cell genetics, fluorescent probe development, mitochondria physiology and cell differentiation and will be an integral part of the recently-established NSF Science and Technology Research Center in Light Microscopy Imaging and Biotechnology at Carnegie Mellon University

这份提案是为了购买荧光灯- 激活细胞分选机，以取代1983年购买的一台， 荧光研究中心成立于 卡内基梅隆大学生物医学系。 的PI 和co-PI是流式细胞术领域的领导者， 目前的流式细胞仪已被用于38个研究出版物 在细胞生物学方面的成就 没有 CMU中存在其他工具。 方面的缺陷 与新仪器相比的能力和困难 维修和保养是更换的理由。 的 一组主要和次要用户收到超过329万美元， 联邦每年提供的直接成本研究支持， 其他机构，包括超过$690，000每年从NSF。 提议的更换仪器将用于 在细胞、分子和生物学领域的研究 发育生物学，包括膜运输，体细胞 细胞遗传学，荧光探针开发，线粒体 生理学和细胞分化，并将成为一个不可或缺的 最近成立的NSF科学和技术 光学显微镜成像与成像研究中心 卡耐基梅隆大学