SGER: Ontogeny of GnRH Expression Vertebrate Neurons

SGER：GnRH 表达脊椎动物神经元的个体发育

• 批准号: 9217837
• 负责人: Andrew Bass
• 金额: $ 4.54万
• 依托单位: Cornell University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-01 至 1993-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9217837&HistoricalAwards=false
• 关键词: SGER; Ontogeny; GnRH; Expression; Vertebrate

Gonadotropin-releasing hormone (GnRH) is a decapeptide that was isolated from brain in 1971. With the development of sophisticated antibody technology, the GnRH neuronal network was demonstrated to be a complex, heterogenous system, comprising three or more discrete neuronal groups and spanning vastly different regions of the brain, including the ganglion of the terminal nerve within the olfactory bulb and nerve, the basal forebrain including the preoptic area, and the caudal diencephalon or midbrain region. Moreover, GnRH neurons project to targets within and outside the brain and serves as both a hormone and a neurotransmitter. It is implicated in controlling the expression and maintenance of neuroendocrinological traits underlying behavioral reproductive mechanisms. This Small Grant for Exploratory Research award to Dr. Bass will facilitate the development of sensitive and specific molecular tools which will be instrumental in providing new insights into the cellular and molecular bases for hormonal control of sexual differentiation of brain and behavior. He will complete the sequence analysis and sequence comparisons of cDNAs for GnRH in a specific species that serves as a model to examine intrasexual dimorphisms in brain and behavior. Dr. Bass will use the antisense ribonucleic acid transcripts generated from the cloned cDNAs and in situ hybridization histochemistry to identify the expression patterns and quantitate levels of novel GnRH transcripts in the brain of juvenile and adult animals with distinct reproductive phenotypes. The development of these new sensitive molecular tools will open up new avenues into this emerging research area. The research will lead to a better understanding of the role that GnRH plays in basic mechanisms underlying reproducive maturation, physiology and species-typical behaviors.

促性腺激素释放激素（GnRH）是一种十肽， 1971年从大脑中分离出来 随着复杂的 抗体技术，GnRH神经元网络被证明， 是一个复杂的，异质的系统，包括三个或更多 离散的神经元群，跨越不同的区域， 大脑，包括神经内的终末神经节， 嗅球和神经，基底前脑，包括 视前区和间脑尾部或中脑区。 此外，GnRH神经元投射到细胞内外的靶点， 大脑中，作为激素和神经递质。 是 参与控制表达和维持 生殖行为的神经内分泌特征 机制等 这一小笔探索性研究奖授予博士。 低音将促进发展的敏感和具体的 分子工具，这将有助于提供新的 深入了解激素控制的细胞和分子基础 大脑和行为的性别差异。 他将完成 促性腺激素释放激素cDNA序列分析与比较 在一个特定的物种，作为一个模型，检查内有性 大脑和行为的二型性 拜斯博士会用反义核酸 从克隆的cDNA产生的核糖核酸转录物， 原位杂交组织化学鉴定表达 新的GnRH转录物的模式和定量水平， 幼年和成年动物的大脑具有不同的生殖功能 表型 这些新的敏感分子工具的发展 将为这一新兴研究领域开辟新的途径。 的 研究将导致更好地了解GnRH的作用， 在生殖成熟的基本机制中发挥作用， 生理学和物种典型行为。