Evolution of the retrotransposons R1 and R2

逆转录转座子 R1 和 R2 的进化

• 批准号: 9219123
• 负责人: Thomas Eickbush
• 金额: $ 35.2万
• 依托单位: University of Rochester
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-02-01 至 1996-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9219123&HistoricalAwards=false
• 关键词: Evolution; retrotransposons; R1; R2

R1 and R2 are sequence-specific retrotransposable elements found inserted in a fraction of the 28S ribosomal RNA genes of most arthropods. The objective of this proposal is to determine whether the remarkable success of these elements is due to their freedom to move from species to species or their ability to remain active in a species lineage over long evolutionary periods. Most experiments will focus on the sequence relationships of R1 and R2 isolated from species of the dipteran genus Drosophila. The 3' half of the elements will be cloned after the polymerase chain reaction amplification and their nucleotide sequences determined. This sequence analysis will be complemented with studies of the distribution of R1 and R2 within different geographical strains of several species using genomic DNA blotting procedures. Experiments will also be conducted with three species of parasitic wasp (genus Nasonia) which contain multiple families of highly divergent R1 elements. %%% The proposed experiments will provide information that is fundamental to an understanding of the stability of mobile elements within genomes, their ability to transfer between species, and their ability to regulate their own abundance.

R1 和 R2 是序列特异性逆转录转座元件，被发现插入大多数节肢动物的 28S 核糖体 RNA 基因的一部分中。 该提案的目的是确定这些元素的显着成功是否是由于它们可以自由地从一个物种转移到另一个物种，或者是因为它们在漫长的进化周期中在一个物种谱系中保持活跃的能力。 大多数实验将集中于从双翅目果蝇属物种中分离的 R1 和 R2 的序列关系。 聚合酶链式反应扩增后，将克隆元件的 3' 部分并确定其核苷酸序列。 该序列分析将与使用基因组 DNA 印迹程序研究 R1 和 R2 在多个物种的不同地理菌株内的分布进行补充。 实验还将使用三种寄生黄蜂（Nasonia 属）进行，它们含有多个高度分化的 R1 元素家族。 %%% 拟议的实验将提供对于了解基因组内移动元件的稳定性、它们在物种之间转移的能力以及它们调节自身丰度的能力至关重要的信息。