RUI: Thermodynamics of Lipid-Lipid Interactions in Model Membranes

这个建议提出了一个计划，研究热力学的 凝胶和液体之间常见脂质膜组分的转移 参考双层，二棕榈酰磷脂酰胆碱， 使用差示扫描量热法作为主要技术。 实验方案涉及在以下条件下进行测量： 双层中溶质组分的非常稀的溶液，和 然后用正规溶液模型计算自由能， 提出用于评价溶质的转移焓， 从而也可以确定熵变化。最后A 完整的热力学描述的转移过程将 允许比较各种脂质类别， 在膜生物物理学中很重要。 通过系统地改变 溶质分子的结构，可以设计一组参数， 将溶质的结构特征与整体 热力学传递特性 分子诸如 磷脂、脂肪酸、鞘脂和类固醇将被 研究了 %%% 生物膜脂质组分间的相互作用 确定的物理和化学特性的 膜中的成分。 热力学评价 这些相互作用对于理解 分子在双层膜环境中。 已经 很难设计出方法来评估热力学 由于膜组件的限制， 可以用来研究相互作用的模型和技术。

This proposal presents a plan to study the thermodynamics of transfer of common lipid membrane components between gel and fluid phases of a reference bilayer, dipalmitoylphosphatidylcholine, using differential scanning calorimetry as the primary technique. The experimental protocols involve carrying out the measurements in very dilute solutions of the solute components in the bilayer, and then using the regular solution model to evaluate the free energy proposed for evaluation of the enthalpy of transfer of the solute, so that the entropy change can also be determined. In the end a complete thermodynamic description of the transfer process will allow comparisons between a variety of lipid classes that are important in membrane biophysics. By systematically varying the structures of solute molecules, a set of parameters can be devised that will relate structural features of the solutes to the overall thermodynamic transfer characteristics. Molecules such as the phospholipids, fatty acids, sphingolipids and steroids will be studied. %%% Interactions between the lipid components of biological membranes determine the physical and chemical characteristics of the components in the membrane. Evaluation of the thermodynamics of these interactions is fundamental to understanding the behavior of molecules in the bilayer membrane environment. It has been difficult to devise methods to evaluate the thermodynamic properties of membrane components because of the limitations of models and techniques that can be used to study the interactions.