Isolation and Characterization of Neural Crest Subpopulation

神经嵴亚群的分离和表征

• 批准号: 9219666
• 负责人: Kate Barald
• 金额: $ 30.17万
• 依托单位: Regents of the University of Michigan - Ann Arbor
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-04-15 至 1997-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9219666&HistoricalAwards=false
• 关键词: Isolation; Characterization; Neural; Crest; Subpopulation

This project, which will begin its tenth year of support from the NSF, addresses one of the critical questions in developmental biology: how do aa relatively small number of embryonic cells give rise to the multitude of cell types that make up the individual? An ideal model system in which this question can be addressed is the neural crest, which appears very early in the development in all vertebrate species. Although the neural crest is only recognizable for a few days during the course of development, it gives rise to a myriad of important structures in the body. Among the hundreds of neural crest derivatives is the entire peripheral nervous system, all of the pigment cells in the skin and the cells that make the dentin in the teeth. How are such an enormous number of diverse cell types made from a seemingly homogeneous population of cells in a normal individual? With previous NSF support, this laboratory devised sophisticated cell sorting techniques for isolating and studying subpopulations of neural crest cells. Furthermore, they can transplant neural crest cells and follow the transplanted cells throughout development. The current studies will specifically examine how individual neuronal cell types arise from the neural crest by examining the development of two identified subpopulations following transplantation. The events that direct apparently homogeneous neural crest cells to become specific neurons will be examined, and the specific control genes that are associated with these events will be identified. These studies are at the interface of cellular and molecular biology, where, for the first time, it is now possible to address one of the most fundamental questions in developmental neuroscience.

该项目将开始第十个年头， 美国国家科学基金会，解决了发展中的一个关键问题， 生物学：相对少量的胚胎细胞是如何产生 形成构成个体的多种细胞类型？ 可以解决这个问题的理想模型系统是 神经嵴，出现在发育的早期， 所有脊椎动物物种。 虽然神经嵴 在开发过程中，可以识别几天， 产生了身体中无数的重要结构。 之间 数百个神经嵴衍生物是整个外周 神经系统，皮肤中的所有色素细胞和细胞 形成牙齿中的牙本质。 如此庞大的数字 从一个看似同质的群体中产生的不同细胞类型 的细胞数量吗 在以前的NSF支持下， 实验室设计了复杂的细胞分选技术， 分离和研究神经嵴细胞的亚群。 此外，他们可以移植神经嵴细胞，并遵循 在整个发育过程中移植细胞。 目前的研究 将专门研究单个神经细胞类型是如何产生的， 通过检查两个细胞的发育，从神经嵴中观察 在移植后鉴定亚群。 的事件 引导明显同质的神经嵴细胞 特定的神经元将被检查，特定的控制基因将被检查， 与这些事件相关的信息将被识别。 这些 研究是在细胞和分子生物学的界面， 在那里，第一次，现在有可能解决其中一个 发展神经科学中最基本的问题