Structure and Function of Regulatory Proteins

建议的研究涉及在NIE-115细胞中表达的兴奋性5-羟色胺3型受体的化学机制。将采用两种快速动力学方法：(1)细胞流动法和(2)激光脉冲光解不稳定的5-羟色胺笼状物质。跨膜通道的行为将在广泛的时间和5-羟色胺浓度范围内进行研究。我们将探索抑制剂的作用。神经递质受体介导的神经系统特定细胞中的反应负责信号传递的启动、调节和调制。这些过程都是神经系统功能所必需的。所提出的研究旨在发展一个完整的动力学理解之间的相互作用的配体(5-羟色胺)和它的受体在选定的细胞膜。根据5-羟色胺是否与受体结合以及结合多长时间，受体形成瞬间的离子传导跨膜通道，提供基本的信号传递事件。为了开发有用的信号传输模型，需要进行仔细的时间相关性研究。这些基本信息的发展是了解神经系统功能和整合本质的基础。

The proposed studies address the chemical mechanism of the excitatory serotonin type-3 receptor as expressed in NIE-115 cells. Two rapid kinetic approaches will be employed: (1) a cell-flow method, and (2) laser-pulse photolysis of labile serotonin cage species. Transmembrane channel behavior will be investigated over wide ranges of time and serotonin concentration. The effects of inhibitors will be explored. %%% Neurotransmitter receptor-mediated reactions in specific cells of the nervous system account for the initiation, regulation, and modulation of signal transmission. These processes are all required for nervous system function. The studies proposed are aimed at developing a complete kinetic understanding of the interaction between a ligand (serotonin) and its receptor in selected cell membranes. Depending on whether serotonin is bound to the receptor and for how long, the receptor forms transient ion- conducting transmembrane channels which provide the fundamental signal transmission event. Careful time-dependence studies are required in order to develop useful models of signal transmission. Development of such basic information is fundamental to progress in understanding the nature of nervous system function and integration.