Hydrogen Tunneling in Enzyme Reactions
酶反应中的氢隧道
基本信息
- 批准号:9221072
- 负责人:
- 金额:$ 24万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Continuing Grant
- 财政年份:1993
- 资助国家:美国
- 起止时间:1993-01-15 至 1995-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Numerous examples exist in the literature documenting the importance of electron tunneling in enzymatic processes. Although much larger than the electron, protium possesses a de Brogile wave length which is similar to the expected dimension of the reaction coordinate in simple H transfer reactions. This property has led to the recognition that the behavior of protium is poised between classical and quantum mechanical behavior. Published studies from this laboratory have demonstrated that quantum effects contribute significantly to several enzyme catalyzed H transfer reactions at room temperature. Ongoing experiments suggest that this phenomenon will be a general one for enzyme catalyzed reactions. A major difficulty in the unambiguous demonstration of tunneling has been the absence of suitable experimental probes. A series of protocols have been developed toward this end: these include the comparative study of H/T and D/T isotope effects and their temperature dependences. Investigations during the next funding period will include the following areas: (1) A number of new enzyme catalyzed H transfer reactions will be examined for tunneling, in order to establish a data base for this phenomenon. In particular, the PI plans to focus on the flavin containing enzymes, monoamine oxidase and glucose oxidase: (2) Studies of the influence on tunneling of specific protein substrate interactions will be pursued through the use of site-specific mutagenesis; and (3) Computational work will be continued, with the goal of reproducing experimental data and providing suitable models for the mechanism of tunneling in enzyme reactions. %%% Although electron tunneling in proteins has been extensively studied, little attention has been paid to nuclear (hydrogen) tunneling. Dr. Klinman's elegant work has shown that nuclear tunneling contributes significantly to certain enzymatic reaction rates under biologically relevant conditions. This project builds and expands upon these groundbreaking observations.
文献中有许多例子证明了电子隧穿在酶促过程中的重要性。尽管质子比电子大得多,但它的德布罗吉尔波长与简单氢转移反应中反应坐标的预期尺寸相似。这一性质使人们认识到,protium的行为介于经典和量子力学行为之间。该实验室发表的研究表明,量子效应在室温下对几种酶催化的H转移反应有重要贡献。正在进行的实验表明,这种现象将是酶催化反应的普遍现象。一个主要的困难在隧道的明确论证是缺乏合适的实验探针。为此制定了一系列方案,其中包括H/T和D/T同位素效应及其温度依赖性的比较研究。下一个资助期的研究将包括以下领域:(1)将对一些新的酶催化的H转移反应进行隧道研究,以便为这种现象建立一个数据库。特别是,PI计划将重点放在含黄素的酶、单胺氧化酶和葡萄糖氧化酶上:(2)将通过使用特定位点诱变来研究对特定蛋白质底物相互作用的隧道化的影响;(3)继续进行计算工作,目的是再现实验数据,为酶反应的隧穿机理提供合适的模型。虽然蛋白质中的电子隧穿已经被广泛研究,但对核(氢)隧穿的关注却很少。Klinman博士优雅的工作表明,在生物相关条件下,核隧道对某些酶促反应速率有显著贡献。这个项目建立并扩展了这些开创性的观察结果。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Judith Klinman其他文献
Emerging Experimental Probes for the Spatial and Temporal Resolution of Protein Dynamics in Enzyme Catalysis
- DOI:
10.1016/j.bpj.2020.11.819 - 发表时间:
2021-02-12 - 期刊:
- 影响因子:
- 作者:
Judith Klinman;Shuaihua Gao;Emily J. Thompson;Jan Paulo Zaragoza - 通讯作者:
Jan Paulo Zaragoza
Judith Klinman的其他文献
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{{ truncateString('Judith Klinman', 18)}}的其他基金
Determining the Effects of Sequence Variation on SARS-CoV-2 Spike Protein through High-Resolution Characterization of Protein Energy Landscapes
通过蛋白质能量景观的高分辨率表征确定序列变异对 SARS-CoV-2 刺突蛋白的影响
- 批准号:
2322801 - 财政年份:2023
- 资助金额:
$ 24万 - 项目类别:
Continuing Grant
Collaborative Research: Mapping and comparing the link of the protein scaffold to quantum events in thermally activated enzymes and flavin-based photoreceptors
合作研究:绘制和比较蛋白质支架与热激活酶和黄素光感受器中量子事件的联系
- 批准号:
2231081 - 财政年份:2023
- 资助金额:
$ 24万 - 项目类别:
Continuing Grant
Linking Protein Dynamics to Hydrogen Tunneling
将蛋白质动力学与氢隧道联系起来
- 批准号:
0446395 - 财政年份:2005
- 资助金额:
$ 24万 - 项目类别:
Continuing Grant
Facility Reconfiguration for Computational Chemistry in Research and Teaching
计算化学研究和教学的设施改造
- 批准号:
0233882 - 财政年份:2003
- 资助金额:
$ 24万 - 项目类别:
Standard Grant
Upgrade of Departmental Multi-User NMR Instrumentation
部门多用户核磁共振仪器升级
- 批准号:
0130862 - 财政年份:2002
- 资助金额:
$ 24万 - 项目类别:
Standard Grant
1996 Gordon Conference: Isotopes in Biology and Chemistry to be held in Ventura, California, February 11-16, 1996
1996年戈登会议:生物学和化学中的同位素将于1996年2月11日至16日在加利福尼亚州文图拉举行
- 批准号:
9527873 - 财政年份:1996
- 资助金额:
$ 24万 - 项目类别:
Standard Grant
相似海外基金
Protein dynamics and hydrogen tunneling in enzyme catalysis
酶催化中的蛋白质动力学和氢隧道
- 批准号:
0715448 - 财政年份:2007
- 资助金额:
$ 24万 - 项目类别:
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Kinetic and X-ray crystallographic analysis of quantum mechanical hydrogen tunneling in enzyme-catalyzed reaction
酶催化反应中量子力学氢隧道的动力学和X射线晶体学分析
- 批准号:
19770118 - 财政年份:2007
- 资助金额:
$ 24万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Tunneling and Dynamics in Enzyme Catalyzed Reactions
酶催化反应中的隧道效应和动力学
- 批准号:
7724848 - 财政年份:2002
- 资助金额:
$ 24万 - 项目类别:
Tunneling and Dynamics in Enzyme Catalyzed Reactions
酶催化反应中的隧道效应和动力学
- 批准号:
9225208 - 财政年份:2002
- 资助金额:
$ 24万 - 项目类别:
Tunneling and Dynamics in Enzyme Catalyzed Reactions
酶催化反应中的隧道效应和动力学
- 批准号:
8089560 - 财政年份:2002
- 资助金额:
$ 24万 - 项目类别:
Tunneling and Dynamics in Enzyme Catalyzed Reactions
酶催化反应中的隧道效应和动力学
- 批准号:
8675525 - 财政年份:2002
- 资助金额:
$ 24万 - 项目类别:
Tunneling and Dynamics in Enzyme Catalyzed Reactions
酶催化反应中的隧道效应和动力学
- 批准号:
8259171 - 财政年份:2002
- 资助金额:
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