Bifunctional Vesicles for Enhancing Elisas

主要研究者（pi）建议开发固相异质酶联免疫吸附测定（elisa）。提出的方法是使用基于磷脂的囊泡（脂质体），其外表面被100到200个酶分子的共价附着修饰，以及一个变量，但更小的，特定的分析物或抗体分子的数量。潜在的信号增强可以通过数百个附着的酶分子来实现，而不是一个或几个，与当前技术相比，将酶联免疫吸附试验的灵敏度限制降低两个数量级。

The Principal Investigators (PIs) propose to develop solid-phase heterogenous enzyme-linked immunosorbent assays (ELISAs). The proposed approach is to use phospholipid-based vesicles (liposomes) whose outer surfaces have been modified by the covalent attachment of 100 to 200 enzyme molecules as well as a variable, but smaller, number of specific analyte or antibody molecules. The potential signal enhancement that could be achieved by hundreds of attached enzyme molecules, rather than one or a few, to the analyte or antibody could result in a reduction of the limit of sensitivity of an ELISA by two orders of magnitude relative to current technology.