Integrated Laboratory Exercises for Undergraduate Cell and Molecular Biology

本科生细胞和分子生物学综合实验室练习

• 批准号: 9250298
• 负责人: William Allen
• 金额: $ 3.18万
• 依托单位: Humboldt State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-06-15 至 1994-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9250298&HistoricalAwards=false
• 关键词: Integrated; Laboratory; Exercises; Undergraduate; Cell

A coordinated set of laboratory core exercises will take upper division cell and molecular biology majors from the cloning of a gene to the purification of its coded protein. The exercises is based on the baculovirus (Bac) expression system, a procedure that inserts foreign genes into a virus which infects cell lines derived from moths and over produces the inserted gene. The laboratory exercises will flow from the subcloning of genes into transfer vectors used with the Bac system to the chromatographic purification of the recombinant protein produced in the tissue cultured moth cells. The DNA to RNA to protein principle of molecular biology is reinforced by the working relationship students receive with such important procedures as: tissue culture, manipulation of viruses of eukaryotic cells, in vitro manipulations of DNA molecules, specific detections of macromolecules (Western, Northern, and Southern blots), and protein purifications. Senior Thesis projects will aslo employ the new laboratory equipment.

一套协调的实验室核心练习将把高级细胞和分子生物学专业的学生从基因的克隆到其编码蛋白质的纯化。这些练习是基于杆状病毒（Bac）表达系统，这是一种将外源基因插入病毒的程序，该病毒感染来自飞蛾的细胞系并过量产生插入基因。实验练习将从基因的亚克隆到与Bac系统一起使用的转移载体，再到组织培养蛾细胞中产生的重组蛋白的层析纯化。分子生物学中DNA到RNA到蛋白质的原理通过学生接受的重要程序的工作关系得到加强，这些程序包括：组织培养、真核细胞病毒的操作、DNA分子的体外操作、大分子的特定检测（西部、北部和南部印迹）和蛋白质纯化。毕业论文项目也将使用新的实验室设备。