The Use of Recombinant DNA Technology in Undergraduate Biology Laboratories

目前，几乎生物学的每一个分支学科都在使用重组DNA技术作为研究工具。随着这项重要技术的出现，生物学专业和非专业本科生获得这项技术的实验室经验至关重要。本计画的目标是在通识生物学课程中，透过一套细菌重组练习，向学生介绍重组DNA的基本实验室经验。重组DNA的经验将在细胞生理学中继续，学生将分离质粒DNA，进行限制性内切酶分析，连接DNA插入物，并进行转化试验。完成一个原创性的研究项目是所有生物学高年级学生的要求。新设备将允许学生参与更广泛的项目，此外，所需设备的获得将允许开发和介绍分子生物学的新课程。

Nearly every subdiscipline in biology is currently using recombinant DNA technology as a research tool. With the advent of this important technology, it is critical that both biology major and non-major undergraduate students gain laboratory experiences in this technology. The goal of this project is to introduce students to basic recombinant DNA laboratory experiences beginning in the General Biology Course employing a set of bacterial recombination exercises. The recombinant DNA experiences will continue in Cell Physiology where the students will isolate plasmid DNA, perform restriction enzyme analyses, ligate DNA inserts, and perform transformation assays. Completion of an original research project is required of all Biology senior students. The new equipment will allow students to participate in a wider variety of projects In addition, acquisition of the requested equipment will allow the development and presentation of a new course in molecular biology.