The Use of Recombinant DNA Technology in Undergraduate Biology Laboratories

重组DNA技术在本科生物实验室中的应用

• 批准号: 9251157
• 负责人: Marilyn Branton
• 金额: $ 2.62万
• 依托单位: Stillman College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-06-01 至 1994-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9251157&HistoricalAwards=false
• 关键词: Use; Recombinant; DNA; Technology; Undergraduate

Nearly every subdiscipline in biology is currently using recombinant DNA technology as a research tool. With the advent of this important technology, it is critical that both biology major and non-major undergraduate students gain laboratory experiences in this technology. The goal of this project is to introduce students to basic recombinant DNA laboratory experiences beginning in the General Biology Course employing a set of bacterial recombination exercises. The recombinant DNA experiences will continue in Cell Physiology where the students will isolate plasmid DNA, perform restriction enzyme analyses, ligate DNA inserts, and perform transformation assays. Completion of an original research project is required of all Biology senior students. The new equipment will allow students to participate in a wider variety of projects In addition, acquisition of the requested equipment will allow the development and presentation of a new course in molecular biology.

几乎生物学的每一个分支学科目前都在使用 重组DNA技术作为研究工具。 来临 这项重要技术的关键是， 专业和非专业本科生获得实验室 在这项技术方面的经验。 该项目的目标是 向学生介绍基本的重组DNA实验室 从普通生物学课程开始， 一系列细菌重组实验 重组 DNA的经验将继续在细胞生理学， 学生将分离质粒DNA，进行限制性内切酶 分析，连接DNA插入物，并进行转化 测定。 完成一个原创性的研究项目是 所有生物学高年级学生的必修课。 新装备 将允许学生参加更广泛的各种 此外，购置所要求的设备 将允许开发和演示新课程， 分子生物学