USE OF RECOMBINANT DNA IN POPULATION GENETICS

重组 DNA 在群体遗传学中的应用

• 批准号: 3279680
• 负责人: ALAN Robert TEMPLETON
• 金额: $ 13.45万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-01-01 至 1986-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3279680
• 关键词: DNA; animal population density; animal population genetics; biological polymorphism; endonuclease; evolution; gene expression; gene frequency; genetic markers; heterozygote; isozymes; mitochondrial DNA; ribosomal DNA; sex chromosomes; species difference

Recombinant DNA technology can be used to assay types of genetic variability in natural populations that were previously not feasible to study. This proposal is concerned with three related applicants of this new ability. The first is the simultaneous use of paternally (Y-chrosomal DNA), maternally (mitochondrial DNA), and bisexually (ribosomal DNA, single copy nuclear DNA) inherited DNA markers to examine the population structure of certain insect and reptile species (although the analytical framework and experimental protocol will also be applicable to studies of human population structure). Population structure refers to the effective population sizes, systems of mating, and patterns of gene flow that occur within a species. These parameters have a major impact on the amount and distribution of genetic variation within a specie and upon the course of adaptive evolution and speciation. However, the current amount and distribution of genetic variation is also dependent upon past events, and most importantly upon founder and bottleneck events in which the species underwent episodes of small population size in the past. Such bottlenecks are thought to have played a critical role in determining the incidence of genetic disease in certain human populations. Consequently, the second application outlined in this proposal will be the reconstruction of historical founder and bottleneck effects from studies on restriction site polymorphism in mitochondrial and ribosomal DNA. The historical information encoded in the DNA also extends back beyond the history of the species itself, and the third application is the use of recombinant DNA techniques in reconstructing phylogenies of closely related existing species. This phylogenetic information is needed to assess the genetic impact of speciation and to more fully integrate population genetics with macroevolution. Such information will also allow us to test many hypotheses stemming from the area of molecular evolution. In summary, recombinant DNA technology will be used to examine some of the evolutionary forces--both current and historical--that greatly influence the amount and distribution of genetic variation among and between populations and species.

重组DNA技术可用于检测基因型， 自然种群的变异性，以前不可行， study. 该提案涉及三个相关申请人， 新能力 第一种是同时使用父系（Y染色体 DNA）、母系（线粒体DNA）和双性（核糖体DNA，单 复制核DNA）遗传DNA标记来检查种群结构 某些昆虫和爬行动物物种（尽管分析框架 和实验方案也将适用于人类的研究 人口结构）。 人口结构是指有效的 种群规模、交配系统和基因流动模式 在一个物种中。 这些参数对数量有重大影响， 遗传变异在一个物种内的分布，以及 适应性进化和物种形成 目前的金额和 遗传变异的分布也取决于过去的事件， 最重要的是，该物种的创始人和瓶颈事件 在过去经历了人口规模小的时期。 这样的瓶颈 被认为在决定 某些人群中的遗传疾病。 因此，第二 本提案中概述的应用程序将是重建 限制性位点研究历史奠基者与瓶颈效应 线粒体和核糖体DNA的多态性。 历史 DNA中编码的信息也可以追溯到人类历史之外。 物种本身，第三个应用是使用重组DNA 重建密切相关的现有系统发育的技术 物种 这种系统发育信息是评估遗传 物种形成的影响，并更充分地将群体遗传学与 宏观进化 这些信息也将使我们能够测试许多 起源于分子进化领域的假说。 总的来说， 重组DNA技术将被用来检查一些进化的 当前和历史上的力量，极大地影响了数量， 遗传变异在种群和物种之间的分布。