Viral Amplification of Recombinant Messenger RNA in Transgenic Plants

转基因植物中重组信使 RNA 的病毒扩增

• 批准号: 9261413
• 负责人: Thomas Turpen
• 金额: $ 5万
• 依托单位: Biosource Technologies Inc
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-01-01 至 1993-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9261413&HistoricalAwards=false
• 关键词: Viral; Amplification; Recombinant; Messenger; RNA

Plants are an inexpensive source of complex molecules. Several industries are based on chemical feedstocks of plant origin, ranging from flavors to pharmaceuticals. The biosynthetic pathways of plants may be more effectively manipulated through viral gene amplification than by conventional genetic engineering. We propose to amplify specific target RNAs in transgenic plants based on viral RNA replication. The 5' and 3' viral replication origins of tobacco mosaic virus will be combined with an internal target sequence to be amplified. Recombinant messenger RNA will be synthesized from a gene inserted into the tobacco chromosome under transcriptional control of a nominally constitutive promoter. In the presence of helper feasibility, we will quantify amplified gene expression using the messenger RNA for the enzyme chloramphenicol acetyltransferase (CAT) as a target sequence. %%% Because the levels of amplification and timing of expression will be controlled by the helper virus, this technique will significantly extend the types of genetic manipulations possible in transgenic crops. Efficient production of high value biochemicals in plants will create new opportunities for domestic growers and strengthen the ability of the agricultural sector of our economy to compete internationally.

植物是复杂分子的廉价来源。 一些工业是基于植物的化学原料 来源，从香料到药品。 生物合成 植物的途径可以更有效地操纵， 病毒基因扩增比传统的基因工程。 我们建议在转基因植物中扩增特定的靶RNA 基于病毒RNA复制。 5'和3'病毒复制 烟草花叶病毒的起源将与一种内部的 待扩增靶序列。 重组信使RNA将被 由插入烟草染色体的基因合成， 转录控制的名义组成型启动子。 在 存在辅助可行性，我们将量化扩增基因 使用氯霉素酶的信使RNA表达 乙酰转移酶（CAT）作为靶序列。 %%% 因为扩增的水平和表达的时机 被辅助病毒控制，这项技术将 大大扩展了基因操作的类型， 转基因作物 高效生产高价值生物化学品 将为国内种植者创造新的机会， 加强我国农业部门的能力， 国际竞争。