Pseudo-Random Modulated Diode Laser for Time Resolved Fluorescence Spectroscopy

用于时间分辨荧光光谱的伪随机调制二极管激光器

• 批准号: 9261484
• 负责人: Hyo Sang Lee
• 金额: $ 4.96万
• 依托单位: Science & Engineering Services Inc
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-01-01 至 1993-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9261484&HistoricalAwards=false
• 关键词: Pseudo; Random; Modulated; Diode; Laser

This proposal pertains to the development of a novel, cost effective, compact sensor for time resolved fluorescence spectroscopy of biological samples. The sensor will have high time resolution (sub-nanosecond) for accurate fluorescence decay time measurements with high sensitivity. The sensor is based on the pseudo random code modulated (PRM) cw lidar technique whereby the fluorescence signal from the PRM laser excitation is detected by a receiver system, and processed using cross correlators to determine the decay profile in real time. The time resolution of the measurement is enhance beyond the quantization precision of the digitizer using a proprietary resolution enhancement technique. The spectral information of the fluorescence signal is obtained using a set of interference filters. Unlike in the pulsed laser fluorescence technique, the detectable signal is not limited by the background noise level due to the inherent characteristic of the PRM phase sensitive technique. In Phase I, we will carry out a proof of concept experiment, critical system analysis, and conceptual system design. This sensor can be used for the in vivo as well as remote measurement of the fluorescence signal from samples in realtime.

该提案涉及开发一种新颖、经济高效、紧凑的传感器，用于生物样品的时间分辨荧光光谱。 该传感器将具有高时间分辨率（亚纳秒），可实现高灵敏度的精确荧光衰减时间测量。 该传感器基于伪随机码调制 (PRM) 连续激光雷达技术，接收器系统可检测来自 PRM 激光激发的荧光信号，并使用互相关器进行处理，以实时确定衰减曲线。 使用专有的分辨率增强技术，测量的时间分辨率超出了数字化仪的量化精度。 使用一组干涉滤光片获得荧光信号的光谱信息。 与脉冲激光荧光技术不同，由于 PRM 相敏技术的固有特性，可检测信号不受背景噪声水平的限制。 在第一阶段，我们将进行概念验证实验、关键系统分析和概念系统设计。 该传感器可用于实时体内和远程测量样品的荧光信号。