Pseudo-Random Modulated Diode Laser for the Time Resolved Fluorescence Spectroscopy

该建议涉及到一个原型的亚纳秒分辨率的光学传感器，利用伪随机调制（PRM）相关技术的时间分辨荧光或光子迁移测量的发展。 第一阶段概念验证实验和伴随的模拟研究的结果清楚地表明了该技术在时间分辨测量方面的强大能力。 在荧光和光子迁移实验中验证了时间分辨增强技术的有效性。 此外，还提出了一种新的方法，用于校正由于信号下降而引起的波形变形，该方法使用的是一个简单的测量泵浦源波形的ad-hoc参考码。 将强大的PRM相关方法与ad-hoc参考代码和分辨率增强技术相结合，现在概念化了一种具有亚纳秒时间分辨率的简单、成本效益高的荧光传感器。 在第二阶段，将开发分辨率为50 psec的光子迁移测量原型传感器，并测试该系统用于测量模拟组织样本的时间分辨反射率，以检索样本的相关参数。 此外，还将研究开发用于PRM相关荧光传感器的可行蓝色激光源。

This proposal pertains to the development of a prototype sub-nanosecond resolution optical sensor utilizing Pseudo-Random Modulation (PRM) Correlation technique for time resolved fluorescence or photon migration measurements. The results of the Phase I proof-of-concept experiment and accompanying simulation studies clearly demonstrated the powerful capabilities of the technique for time resolved measurements. The validity of the Time Resolution Enhancement Technique was verified in both fluorescence and photon migration experiments. In addition, a new method for correcting waveform deformation due to the signal droop, is developed by using an ad-hoc reference code which is simply the measured pump source waveform. Combining the powerful PRM-Correlation method with the ad-hoc reference code and the Resolution Enhancement Technique, a simple, cost effective fluorosensor with sub-nanosecond time resolution is now conceptualized. In Phase II, a prototype sensor for photon migration measurement with 50 psec resolution will be developed, and the system will be tested for the measurement of time resolved reflectance from a simulated tissue sample to retrieve the relevant parameters of the sample. In addition, research for development of viable blue laser sources for PRM-Correlation fluorosensor will also be performed.