Pseudo-Random Modulated Diode Laser for the Time Resolved Fluorescence Spectroscopy

这项建议涉及利用伪随机调制(PRM)相关技术开发用于时间分辨荧光或光子迁移测量的亚纳秒分辨率光学传感器的原型。第一阶段概念验证实验和伴随的模拟研究的结果清楚地表明了该技术在时间分辨测量方面的强大能力。荧光和光子迁移实验验证了时间分辨率增强技术的有效性。此外，还提出了一种新的校正由于信号下垂引起的波形变形的方法，该方法使用一个特别参考码，该参考码简单地是测量的泵浦源波形。结合强大的PRM相关方法、特别参考码和分辨率增强技术，一种简单、经济、具有亚纳秒时间分辨率的荧光传感器现已概念化。在第二阶段，将开发一个分辨率为50PSEC的光子迁移测量原型传感器，并将测试该系统以测量模拟组织样品的时间分辨反射率，以提取样品的相关参数。此外，还将研究开发用于PRM相关荧光传感器的可行的蓝色激光光源。

This proposal pertains to the development of a prototype sub-nanosecond resolution optical sensor utilizing Pseudo-Random Modulation (PRM) Correlation technique for time resolved fluorescence or photon migration measurements. The results of the Phase I proof-of-concept experiment and accompanying simulation studies clearly demonstrated the powerful capabilities of the technique for time resolved measurements. The validity of the Time Resolution Enhancement Technique was verified in both fluorescence and photon migration experiments. In addition, a new method for correcting waveform deformation due to the signal droop, is developed by using an ad-hoc reference code which is simply the measured pump source waveform. Combining the powerful PRM-Correlation method with the ad-hoc reference code and the Resolution Enhancement Technique, a simple, cost effective fluorosensor with sub-nanosecond time resolution is now conceptualized. In Phase II, a prototype sensor for photon migration measurement with 50 psec resolution will be developed, and the system will be tested for the measurement of time resolved reflectance from a simulated tissue sample to retrieve the relevant parameters of the sample. In addition, research for development of viable blue laser sources for PRM-Correlation fluorosensor will also be performed.