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CRB: Mitochondrial DNA Polymorphism in Plant Populations: Analysis of Subdivision and Development of Generalized PCR Methodology

CRB：植物群体线粒体DNA多态性：广义PCR方法学的细分分析和发展

基本信息

批准号：
9300083
负责人：
Steven Strauss
金额：
$ 21.8万
依托单位：
Oregon State University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
1993
资助国家：
美国
起止时间：
1993-08-01 至 1999-01-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=9300083&HistoricalAwards=false
关键词：
CRB Mitochondrial DNA Polymorphism Plant

项目摘要

9300083 Strauss The goal of this research is to test the usefulness of mitochondrial DNA as a marker of population differentiation in plants. Preliminary studies of mitochondrial DNA variation in pines have indicated that very strong differentiation can exist in the presence of weak nuclear gene (allozyme) differentiation. This observation will be rigorously examined via nuclear and mitochondrial DNA-RFLP analysis using cloned and PCR-derived hybridization probes, respectively. A marker that is highly sensitive to population differences could aid conservation biologists when choosing among populations for inclusion in a conservation program--as well as have many other uses in plant population biology and breeding. %%% The study will employ the California Closed-Cone Pines (Pinus attenuata, P. muricata, and P. radiata) as a model system. This group has been the subject of extensive population genetic and systematic studies over several decades--most recently for allozyme, chloroplast DNA, and coxI mitochondrial DNA polymorphism. %%% If time permits, work will begin to develop methods that rely exclusively on PCR for studying mitochondrial DNA polymorphism. Such a method would be as easy to conduct as isozyme studies, and thus increase the extent to which operational conservation programs could use mitochondrial DNA studies in identifying populations for protection or sampling. uct as isozyme studies, and thus increase the extent to which operational conservation programs could use mitochondrial DNA studies in identifying populatio ns for protection 1 - / 1 ! ! ! ! h ! F 1 1 ( Times New Roman Symbol & Arial " h N N - Gayle Edmonds Gayle Edmonds

小行星9300083 本研究的目的是测试线粒体DNA作为植物群体分化标记的有用性。对松树线粒体DNA变异的初步研究表明，在核基因（等位酶）分化较弱的情况下，也可能存在很强的分化。将分别使用克隆和PCR衍生的杂交探针通过核和线粒体DNA-RFLP分析严格检查这一观察结果。一个对种群差异高度敏感的标记可以帮助保护生物学家在种群中选择纳入保护计划-以及在植物种群生物学和育种中有许多其他用途。本研究将采用加州闭锥松（Pinus attenuata、P. muricata和P. radiata）作为模型系统。这一组已被广泛的人口遗传学和系统的研究了几十年-最近的等位酶，叶绿体DNA和coxI线粒体DNA多态性的主题。 %如果时间允许，将开始开发完全依靠PCR研究线粒体DNA多态性的方法。这种方法将像同工酶研究一样容易进行，从而增加了可操作的保护计划在确定保护或采样种群时使用线粒体DNA研究的程度。乌特阿斯同工酶的研究，从而增加的程度，可操作的保护计划可以使用线粒体 DNA研究在确定受保护人群中的应用 1 - / 1 ! ! ! ! H ! F 1 1 ( Times New Roman 符号Arial " H N N - 盖勒埃德蒙兹盖勒埃德蒙兹