CRB: Mitochondrial DNA Polymorphism in Plant Populations: Analysis of Subdivision and Development of Generalized PCR Methodology

本研究的目的是测试线粒体DNA作为植物群体分化标记的有效性。对松木线粒体DNA变异的初步研究表明，在弱核基因（等位酶）分化的情况下，可能存在很强的分化。这一观察结果将通过核和线粒体DNA-RFLP分析，分别使用克隆和pcr衍生的杂交探针进行严格检验。一个对种群差异高度敏感的标记可以帮助保护生物学家在种群中选择纳入保护计划的种群，以及在植物种群生物学和育种中有许多其他用途。该研究将采用加利福尼亚闭锥松（衰减松、村松和辐射松）作为模型系统。几十年来，这一群体一直是广泛的群体遗传和系统研究的主题——最近的研究对象是同工酶、叶绿体DNA和辅酶线粒体DNA多态性。如果时间允许，将开始开发完全依靠PCR来研究线粒体DNA多态性的方法。这种方法与同工酶研究一样容易进行，从而增加了操作保护计划可以使用线粒体DNA研究来确定保护或采样的种群的程度。作为同工酶的研究，从而增加了操作性保护项目利用线粒体DNA研究来确定需要保护的种群的程度- / 1!!!!h !F 11 (Times New Roman Symbol & Arial " h N N - Gayle Edmonds

9300083 Strauss The goal of this research is to test the usefulness of mitochondrial DNA as a marker of population differentiation in plants. Preliminary studies of mitochondrial DNA variation in pines have indicated that very strong differentiation can exist in the presence of weak nuclear gene (allozyme) differentiation. This observation will be rigorously examined via nuclear and mitochondrial DNA-RFLP analysis using cloned and PCR-derived hybridization probes, respectively. A marker that is highly sensitive to population differences could aid conservation biologists when choosing among populations for inclusion in a conservation program--as well as have many other uses in plant population biology and breeding. %%% The study will employ the California Closed-Cone Pines (Pinus attenuata, P. muricata, and P. radiata) as a model system. This group has been the subject of extensive population genetic and systematic studies over several decades--most recently for allozyme, chloroplast DNA, and coxI mitochondrial DNA polymorphism. %%% If time permits, work will begin to develop methods that rely exclusively on PCR for studying mitochondrial DNA polymorphism. Such a method would be as easy to conduct as isozyme studies, and thus increase the extent to which operational conservation programs could use mitochondrial DNA studies in identifying populations for protection or sampling. uct as isozyme studies, and thus increase the extent to which operational conservation programs could use mitochondrial DNA studies in identifying populatio ns for protection 1 - / 1 ! ! ! ! h ! F 1 1 ( Times New Roman Symbol & Arial " h N N - Gayle Edmonds Gayle Edmonds