Analysis of Mechanisms in Stimulous-Secretion Coupling

刺激-分泌耦合机制分析

• 批准号: 9300799
• 负责人: Joel Keizer
• 金额: $ 25.68万
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-07-15 至 1996-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9300799&HistoricalAwards=false
• 关键词: Analysis; Mechanisms; Stimulous; Secretion; Coupling

WPC 2 B R V ` Z #| x Courier 10cpi 2 x x x , x @ 8 ; X @ HP Laserjet (25-in-one, Network) HPLASEII.PRS x @ , t 0 NX @ 2 B ~ # x 6 X @ 8 ; X @# ( 9300799 Keizer The long term objective of this project is to understand the molecular mechanisms that control secretion of hormones from cells in response to various types of stimulatory events. These mechanisms, which are currently being uncovered by experimentalists, least to complex phenomena involving oscillations and waves that require the use of mathematical and computational analysis for their understanding. Of particular interest are 1) the mechanisms controlling calcium oscillations and waves in secretory cells; 2) the mechanism involved in agonist stimulation of G protein events, especially, control of ion channels in the pancreatic beta cell; 3) the mechanisms underlying the 2 5 minute period calcium oscillations in insulin secretion from pancreatic secreting cells that are under the control of glucose metabolism; 4) the mechanisms involved in the control of electrical activity and calcium levels in anterior pituitary gonadotrophs; and 5) the mechanisms responsible for the 15 30 period oscillations in insulin secretion from pancreatic islets in vitro. The method employed by the principal investigator involve the development and analysis of ordinary and partial differential equations corresponding to mechanisms uncovered by experiment. Sufficient quantitative information regarding the rates of these five mechanisms is available to justify these calculations, which will continue to be interfaced closely with experimental work. The computational work will be done on a parallel architecture platform. This proposal is being jointly supported by the Cell Biology, the Computational Biology, and the Applied Mathematics programs. ***

WPC 2 B R V ` Z #|X 10cpi 2 x x x， X @ 8 ; X @ HP Laserjet（25合一，网络） HPLASEII.PRS X @ , 的t0 NX @ 2 B ~ # X 6 X @ 8个; X @# ( 小行星9300799 本项目的长期目标是了解控制细胞分泌激素以响应各种类型的刺激事件的分子机制。 这些机制，这是目前正在发现的实验学家，至少涉及复杂的现象，需要使用数学和计算分析，他们的理解振荡和波。 特别感兴趣的是1）控制分泌细胞中钙振荡和波动的机制; 2）涉及G蛋白事件的激动剂刺激的机制，特别是胰腺β细胞中离子通道的控制; 3）在葡萄糖代谢控制下的胰腺分泌细胞的胰岛素分泌中2 - 5分钟周期钙振荡的潜在机制; 4）垂体前叶促性腺激素细胞电活动和钙离子水平的控制机制; 5）体外胰岛分泌15 ~ 30周期振荡的机制。 主要研究者所采用的方法涉及与实验发现的机制相对应的常微分方程和偏微分方程的开发和分析。 关于这五种机制的速率，有足够的定量信息可以证明这些计算是合理的，这些计算将继续与实验工作密切结合。 计算工作将在并行架构平台上完成。 该提案得到了细胞生物学，计算生物学和应用数学计划的共同支持。***