Controls on the Isotopic Compositions of Individual Compoundin Photosynthethetic Autotrophs

本提案的目的是研究以下方面的重要性： 环境变量对主要的同位素组成的影响 浮游植物中的生物化学成分。 具体而言是 目的是更好地了解营养和光- 局限性影响了 光合自养生物中的单个有机化合物。的 研究将评估营养限制的影响， 主要藻类生物标志物的代表物种。完成 这种主要藻类的代表性物种（即， 硅藻纲、Prymnesiophyceae、蓝藻纲和甲藻纲） 将在各种条件下生活恒化器生长室 它们的同位素是用来测定浮游动物 吃草的结构和同位素组成的脂质和 颜料. 这将通过允许浮游植物 来自一个恒化器生长室的原料流到 控制条件进入含有浮游动物的第二个腔室。 藻类生化成分将与 浮游动物来研究微生物链的相互作用。 研究 包括降解对藻类同位素组成的影响， 生物标志物。由于食草动物是最重要的'水槽'， 自养生产，结构/同位素知识 在这个过程中的变化是至关重要的解释， 藻类降解的分布和同位素组成 沉积有机质中的产物。 基线 这项研究提供的信息需要更好地解释 脂质和色素的同位素组成分布 颗粒物和沉积有机物。 这些信息将 最终可以确定源生物， 许多生物化学品，并取得重大进展， 确定推动有机碳再循环的过程， 海洋

The goal of this proposal is to investigate the importance of environmental variables on the isotopic composition of major component biochemicals in phytoplankton. Specifically, the objective is to better understand how nutrient and light- limitations affects distributions and isotopic compositions of individual organic compounds in photosynthetic autotrophs. The study will assess the affects of nutrient limitation and representative species of the major algal biomarkers. To accomplish this, representative species of the major algal classes (i.e., Bacillariophyceace, Prymnesiophyceae, Cyanophyceae and Dinophyceae) will live chemostat growth chambers under a variety of conditions and their isotopic is to determine the effect of zooplankton grazing on the structure and isotopic compositions of lipids and pigments. This will be accomplished by allowing phytoplankton feedstock from one chemostat growth chamber to flow under controlled conditions into a second chamber containing zooplankton. Composition of algal biochemical will be compared with those of the zooplankton to investigate microbial chain interactions. The study includes degradation effects on isotopic compositions of algal biomarkers. Since herbivory is the most important 'sink' for autotrophic production, knowledge of structural/isotopic alterations during this process is critical for interpreting the distributions and isotopic compositions of algal degradation products in sinking and sedimentary organic matter. The baseline information provided by this research is needed to better interpret distributions of isotopic composition of lipids and pigments in particulate and sedimentary organic matter. Such information will ultimately make it possible to determine the source organisms for numerous biochemicals and to make significant advances in identifying processes driving recycling of organic carbon in the ocean.