Role of Agrobacterium VirB Proteins in T-DNA Transfer

农杆菌 VirB 蛋白在 T-DNA 转移中的作用

• 批准号: 9306482
• 负责人: Lois Banta
• 金额: $ 1.8万
• 依托单位: Haverford College
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-09-01 至 1995-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9306482&HistoricalAwards=false
• 关键词: Role; Agrobacterium; VirB; Proteins; DNA

This is a study of the mechanism of bacterial infection of susceptible plants. Integration of the Agrobacterium tumefaciens T-DNA into the plant genome results in characteristic pathology. Bacterial infection involves transfer of T-DNA and some proteins from the bacterium to the plant. T-DNA and virE2 protein transfer is mediated by proteins which are products of the bacterium virB operon. Studies planned are aimed at: (1) characterizing the behavior of the virB proteins, particularly the possible formation of membrane-associated aggregates. Cross-linking approaches will be employed. The expression stoichiometries of the virB proteins will be examined. The roles of individual virB proteins in the transport process will be evaluated via mutations of individual virB genes. (2) The transport mechanism itself will be examined through use of chemically modified or mutagenically modified virE2 protein, which may interfere with the transport mechanism. %%% Plant pathogens play an important role in the dynamics of the planetary biomass. More specifically, these pathogens affect ornamental as well as agriculturally important species. Therefore, beyond the intrinsic interest in the assembly of a complex system to transfer genetic information from the bacterium to the plant, the characterization of the infection process may lead to development of selective intervention strategies with economic and environmentally positive consequences. The studies planned utilize biochemical and genetic approaches to characterize the mechanisms by which bacteria infect susceptible plants.

这是对感病植物细菌侵染机理的研究。将根癌农杆菌T-DNA整合到植物基因组中，导致了特有的病理。细菌感染包括T-DNA和一些蛋白质从细菌转移到植物。T-DNA和VirE2蛋白的转移是由细菌VIRB操纵子的产物蛋白质介导的。计划的研究目标是：(1)表征VIRB蛋白的行为，特别是可能形成的膜相关聚集体。将采用交叉链接的方法。将检测VIRB蛋白的表达化学计量学。单个VIRB蛋白在运输过程中的作用将通过单个VIRB基因的突变来评估。(2)通过使用化学修饰或突变修饰的VirE2蛋白来检测转运机制本身，这可能会干扰转运机制。植物病原菌在地球生物量的动态变化中起着重要作用。更具体地说，这些病原体影响到观赏和农业上重要的物种。因此，除了组装复杂的系统将遗传信息从细菌转移到植物的内在兴趣之外，感染过程的特征可能导致开发具有经济和环境积极影响的选择性干预策略。计划中的研究利用生化和遗传方法来表征细菌感染敏感植物的机制。