Agrobacterium vitis专门侵染葡萄并能引起根瘤病，是世界各地葡萄生产上最具危害性的病原菌。无致瘤性的A. vitis 菌株F2/5能特异性地抑制致瘤性强的A. vitis菌株对嫁接伤口的侵染，却不影响嫁接苗的生长发育。本项目将在前期工作基础上深入研究 F2/5 抑瘤的分子机制，探索F2/5、葡萄伤口信号分子以及病原菌的毒性基因表达调控之间的关系; 利用分子手段结合表型分析，验证 F2/5 的抑瘤活性与病原菌的 virA 启动子区之间的关系；通过实时荧光PCR及 UPLC-DAD-QTOF-MS等分析手段，研究 F2/5 是否通过干扰葡萄伤口组织某些酚类化合物的产生进而抑制 A. vitis毒性基因的表达。本研究将揭示F2/5抑制葡萄根瘤病的分子机制，为 F2/5 在实际生产中的应用打下基础，对进一步理解葡萄—根瘤菌的互作机制也具有重要意义。

Agrobacterium vitis specifically infects grapevines and causes crown gall, which is the most destructive pathogen threatening grape production worldwide. A non-tumorigenic A. vitis strain F2/5 specifically inhibits the infection of graft wounds by highly tumorigenic A. vitis strains, while has no impacts on graft development. In this research we will further investigate the molecular mechanisms involved in the tumor inhibition (TI) activity of F2/5, and explore the interactions among F2/5, grapevine wound signaling molecules and the regulation of virulence gene expression in the pathogen. One approach is to verify the association between the TI activity of F2/5 and the virA promoter region of the pathogen by molecular approaches and phenotypic characterization. Another approach is to investigate whether F2/5 interferes with the production of certain phenolic compounds at grapevine wound tissues thereby inhibits the expression of virulence genes in A. vitis through real-time qPCR and UPLC-DAD-QTOF-MS analysis. This research will unveil the molecular mechanisms of F2/5 involved in specific inhibition of grape crown gall caused by A. vitis, which is critical for the future application of F2/5. It is also helpful in advancing our understanding of grapevine-Agrobacterium interactions.