The Biochemical and Genetic Comparisons of Novel B Galactosidases

新型 B 型半乳糖苷酶的生化和遗传学比较

• 批准号: 9306764
• 负责人: Jean Brenchley
• 金额: $ 4.95万
• 依托单位: Pennsylvania State Univ University Park
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-06-01 至 1994-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9306764&HistoricalAwards=false
• 关键词: Biochemical; Genetic; Comparisons; Novel; Galactosidases

This project is centered on studies of soil microorganisms growing at relatively low temperatures (five degrees centigrade). The properties of the enzyme betagalactosidase are being studied in these organism in order to understand how protein structure dictates the temperature optimum of an enzyme's activity. The genes encoding these activities are being cloned from Arthrobacter strains and compared with information found for other microorganisms with higher growth temperatures. Creative selection procedures are also being developed to isolate microorganisms with other novel betagalactosidases. %%% The dominant environment of the earth and its oceans is below twenty degrees centigrade, and there is a rich source of diverse microorganisms which have evolved to grow in the cold. Enzymes active at low temperatures could be used in food processing, bioremediation, and molecular biology.

该项目的核心是研究土壤微生物的生长 在相对较低的温度下（5摄氏度）。 的 β-半乳糖苷酶的性质正在研究中， 为了了解蛋白质结构 决定了酶活性的最适温度。 的 编码这些活性的基因正在从节杆菌中克隆出来 菌株，并与其他菌株的信息进行比较 微生物生长温度较高。 创意选择 还正在开发分离微生物的程序， 其他新的β-半乳糖苷酶。 %%% 地球及其海洋的主要环境是在 二十摄氏度，有一个丰富的来源， 微生物已经进化到可以在寒冷中生长。 酶 在低温下具有活性，可用于食品加工， 生物修复和分子生物学。