Strategy for Increasing Yield in Aerobic Fermentations

9309759韦伯斯特当来自透明颤菌的血红蛋白基因(Vgb)存在于携带嗜热脂肪芽孢杆菌α-淀粉酶基因的重组大肠杆菌中时，这种酶的产生被刺激。这个项目的主要目标是获得关于这种刺激机制的基本信息。Vgb的表达产物透明颤菌血红蛋白(VtHb)的作用将通过定点突变来检测，以产生失活和改变的蛋白质。然后，这些不活跃和改变的蛋白质将被测试刺激效应的变化。VtHb在低氧条件下增加，推测在这些条件下通过促进呼吸发挥作用。其他计划中的实验包括将基本发现扩展到除α-淀粉酶之外的其他产品，以及其他细菌系统。

9309759 Webster When the hemoglobin gene (vgb) from the bacterium, Vitreoscilla, is present in recombinant E. coli carrying the gene for alpha- amylase from Bacillus stearothermophilus, the production of this enzyme is stimulated. The main objective of this project is to obtain basic information about the mechanism of this stimulation. The role of the product of vgb expression, Vitreoscilla hemoglobin (VtHb), will be examined using site-directed mutagenesis to produce inactive and altered proteins. These inactive and altered proteins will then be tested for a change in the stimulatory effect. VtHb increases under hypoxic conditions and presumably functions by facilitating respiration under these conditions. Other planned experiments include extending the basic findings to other products besides alpha-amylase, and to other bacterial systems.