Strategy for Increasing Yield in Aerobic Fermentations

9309759 韦伯斯特当来自细菌玻璃球菌的血红蛋白基因（vgb）存在于重组E.携带嗜热脂肪芽孢杆菌α-淀粉酶基因的大肠杆菌中，这种酶的产生被刺激。 本项目的主要目标是获得有关这种刺激机制的基本信息。 vgb表达的产物，玻璃体血红蛋白（VtHb）的作用，将使用定点诱变产生无活性和改变的蛋白质进行检查。 然后将测试这些失活和改变的蛋白质的刺激作用的变化。 VtHb在缺氧条件下增加，并推测在这些条件下通过促进呼吸而起作用。 其他计划的实验包括将基本发现扩展到除α-淀粉酶之外的其他产物和其他细菌系统。

9309759 Webster When the hemoglobin gene (vgb) from the bacterium, Vitreoscilla, is present in recombinant E. coli carrying the gene for alpha- amylase from Bacillus stearothermophilus, the production of this enzyme is stimulated. The main objective of this project is to obtain basic information about the mechanism of this stimulation. The role of the product of vgb expression, Vitreoscilla hemoglobin (VtHb), will be examined using site-directed mutagenesis to produce inactive and altered proteins. These inactive and altered proteins will then be tested for a change in the stimulatory effect. VtHb increases under hypoxic conditions and presumably functions by facilitating respiration under these conditions. Other planned experiments include extending the basic findings to other products besides alpha-amylase, and to other bacterial systems.