Dissertation Research: Conservation Genetics of Isotria medeoloides (Orchidaceae)

论文研究：Isotria medeoloides（兰科）的保护遗传学

• 批准号: 9311268
• 负责人: Christopher Campbell
• 金额: $ 0.45万
• 依托单位: University of Maine
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-08-01 至 1996-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9311268&HistoricalAwards=false
• 关键词: Dissertation; Research; Conservation; Genetics; Isotria

9311268 Campbell Genetic variation of Isotria medeoloides a rare and endangered species of North American orchid, is unknown. The investigators propose to use Random Amplified Polymorphic DNA (RAPD), which permits minimally destructive sampling of the plants, to explore the population structure of this species. To test a possible link between rarity and genetic variability, they will contrast percent variable (polymorphic) loci between six populations of this rare orchid with that of six populations of the only other species in the genus. To see whether population size affects genetic variation, three large and three small populations for both species will be compared. %%% Knowledge of the population genetics of this species would better enable managers of this Federally Endangered orchid to meet longterm recovery goals, ascertain relative importance of the populations, define a minimum viable population size, and measure changes in total diversity over time.

9311268 Campbell Isotria medeoloides 是北美兰花的稀有濒危物种，其遗传变异尚不清楚。 研究人员建议使用随机扩增多态性 DNA (RAPD) 来探索该物种的种群结构，该技术允许对植物进行破坏性最小的采样。 为了测试稀有性和遗传变异之间可能存在的联系，他们将比较这种稀有兰花的六个种群与该属中唯一其他物种的六个种群之间的可变（多态）基因座百分比。 为了了解种群大小是否影响遗传变异，将对两个物种的三个大种群和三个小种群进行比较。 %%% 了解该物种的种群遗传学将更好地帮助该联邦濒危兰花的管理者实现长期恢复目标，确定种群的相对重要性，定义最小可行种群规模，并测量总多样性随时间的变化。