Dissertation Research: Conservation Genetics of Isotria medeoloides (Orchidaceae)

论文研究：Isotria medeoloides（兰科）的保护遗传学

• 批准号: 9311268
• 负责人: Christopher Campbell
• 金额: $ 0.45万
• 依托单位: University of Maine
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-08-01 至 1996-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9311268&HistoricalAwards=false
• 关键词: Dissertation; Research; Conservation; Genetics; Isotria

9311268 Campbell Genetic variation of Isotria medeoloides a rare and endangered species of North American orchid, is unknown. The investigators propose to use Random Amplified Polymorphic DNA (RAPD), which permits minimally destructive sampling of the plants, to explore the population structure of this species. To test a possible link between rarity and genetic variability, they will contrast percent variable (polymorphic) loci between six populations of this rare orchid with that of six populations of the only other species in the genus. To see whether population size affects genetic variation, three large and three small populations for both species will be compared. %%% Knowledge of the population genetics of this species would better enable managers of this Federally Endangered orchid to meet longterm recovery goals, ascertain relative importance of the populations, define a minimum viable population size, and measure changes in total diversity over time.

9311268同位素梅洛洛伊德斯的坎贝尔遗传变异是北美兰花的一种罕见且濒危的物种，尚不清楚。 研究人员建议使用随机扩增的多态性DNA（RAPD），该多态性DNA（RAPD）允许对植物的最小破坏性采样，以探索该物种的种群结构。 为了测试稀有性与遗传变异性之间的可能联系，它们将与该稀有兰花的六个种群与该属中唯一的其他物种的六个种群之间的六个种群对比。 为了查看人口规模是否影响遗传变异，将比较三个物种的三个大和三个小种群。 %% %%对该物种的人口遗传学的了解将使这种受联邦濒危兰花的经理能够实现长期恢复目标，确定人口的相对重要性，定义最小可行的人口规模，并衡量随着时间的推移总体多样性的变化。