Dissertation Research: Conservation Genetics of Isotria medeoloides (Orchidaceae)

9311268坎贝尔 北美珍稀濒危兰科植物--地中海Isotria medeoloides的遗传变异尚不清楚。 研究人员建议使用随机扩增多态性DNA（RAPD），它允许对植物进行最低限度的破坏性取样，以探索该物种的种群结构。 为了测试稀有性和遗传变异性之间可能存在的联系，他们将这种稀有兰花的六个种群与该属中唯一其他物种的六个种群之间的可变（多态）位点百分比进行比较。 为了了解种群大小是否影响遗传变异，将对两个物种的三个大种群和三个小种群进行比较。 该物种的种群遗传学知识将更好地使这一联邦濒危兰花的管理人员，以满足长期的恢复目标，确定人口的相对重要性，定义一个最小可行的人口规模，并衡量随着时间的推移总的多样性变化。

9311268 Campbell Genetic variation of Isotria medeoloides a rare and endangered species of North American orchid, is unknown. The investigators propose to use Random Amplified Polymorphic DNA (RAPD), which permits minimally destructive sampling of the plants, to explore the population structure of this species. To test a possible link between rarity and genetic variability, they will contrast percent variable (polymorphic) loci between six populations of this rare orchid with that of six populations of the only other species in the genus. To see whether population size affects genetic variation, three large and three small populations for both species will be compared. %%% Knowledge of the population genetics of this species would better enable managers of this Federally Endangered orchid to meet longterm recovery goals, ascertain relative importance of the populations, define a minimum viable population size, and measure changes in total diversity over time.