Molecular Genetics of Amino Acid Biosynthesis in Arabidopsis

拟南芥氨基酸生物合成的分子遗传学

• 批准号: 9317175
• 负责人: Gerald Fink
• 金额: $ 88.4万
• 依托单位: Whitehead Institute for Biomedical Research
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-02-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9317175&HistoricalAwards=false
• 关键词: Molecular; Genetics; Amino; Acid; Biosynthesis

Abstract Fink This proposal will identify the specific genes involved in pathway of IAA biosynthesis, the tissue specificity of these genes, the phenotypes of mutations that prevent expression, and regulatory mutations affecting the pathway. The plant hormone, indole acetic acid (IAA) has remarkably diverse effects on plant growth and morphogenesis (22) (e.g. apical dominance, cell division, cell enlargement and lateral root formation). Our belief is that once the gene(s) specifically involved in IAA biosynthesis are cloned, the role of internal IAA in each of these processes can be understood. As most of the structural genes in the pathway are found in multiple copies, mutations will be sought in each member of a set so that the role of gene redundancy in the tryptophan/IAA pathway can be determined. To achieve this goal experiments are designed to improve transformation, gene targeting, and the control of gene expression by antisense. %%% Indole acetic acid is a plant hormone which affects many growth and morphogenic characteristics of plants. Most of what is known have been determined by external application of IAA to plants. However, little is known about its synthesis and function in an in vivo situation. The aromatic amino acid pathway is utilized for the biosynthesis of IAA and we will identify and clone the genes encoding the enzymes of this pathway, determine gene copy number and attempt to gain insight into the internal functioning of the hormone. We will, as necessary, develop the technology needed to achieve these goals. ***

本课题将确定IAA生物合成途径中涉及的特定基因、这些基因的组织特异性、阻止表达的突变表型以及影响该途径的调控突变。植物激素吲哚乙酸（IAA）对植物生长和形态发生的影响具有显著的多样性（22）（如顶端优势、细胞分裂、细胞扩大和侧根形成）。我们相信，一旦特异性参与IAA生物合成的基因被克隆，内部IAA在这些过程中的作用就可以被理解。由于该通路中的大多数结构基因在多个拷贝中被发现，因此将在一组中的每个成员中寻找突变，以便确定基因冗余在色氨酸/IAA通路中的作用。为了实现这一目标，实验旨在改善转化，基因靶向，并通过反义控制基因表达。吲哚乙酸是一种影响植物许多生长和形态发生特性的植物激素。已知的大部分是由植物外用IAA确定的。然而，人们对其在体内的合成和功能知之甚少。芳香氨基酸途径是IAA的生物合成途径，我们将鉴定和克隆该途径酶的编码基因，确定基因拷贝数，并试图深入了解激素的内部功能。如有必要，我们将开发实现这些目标所需的技术。＊＊＊