Acquisition of 200 KeV Transmission Electron Microscope

购置200 KeV透射电子显微镜

• 批准号: 9317781
• 负责人: William Greenough
• 金额: $ 26.64万
• 依托单位: University of Illinois at Urbana-Champaign
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-06-15 至 1997-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9317781&HistoricalAwards=false
• 关键词: Acquisition; 200; KeV; Transmission; Electron

The purpose of this proposal is to request funds for the purchase of a Philips CM- 200 Transmission Electron Microscope to be housed in the Visualization Facility (BVF) of the Beckman Institute on the University of Illinois campus. The Principal Investigators will be using this electron microscope for a variety of studies including (i) neuroplasticity and its relationship to underlying cellular and system level processes (William Greenough), (ii) the study of monodisperse rod-coil block copolymers (Samuel Stupp), (iii) analysis of nuclear and chromosome architecture (Andrew Belmont), (iv) sex differences in the organization and behavioral function of the brain (Janice Juraska), and (v) the characterization of morphological and functional abnormalities in cerebellar neural circuitry in a genetic animal model of movement dysfunction (Louise Abbott). In addition to these core projects there are 6 other research projects from a variety of disciplines for which this instrument provides immediate or long term benefits. The purpose of obtaining a new instrument is twofold. Firstly it will provide access to an intermediate voltage instrument for biological research on the UIUC campus. The only intermediate voltage instruments in this region are exclusively devoted to material science research and biologists with projects for which higher voltages are necessary have to travel to remote sites. Secondly it will replace an existing 18 year old instrument currently located in the BVF that is suffering increasingly from age related problems. In addition to providing unique capabilities for imaging thicker specimens this microscope will have a number of other important advantages over the existing instrument. The ability to control the instrument through a computer interface and to collect micrographs using a CCD camera will greatly aid, and in certain cases be essential for several applications. Specifically, for techniques such as serial sectioning, quantitative stereology and single axis tomography which involve the collection or examination of large numbers of images, semi-automatic acquisition procedures can be devised that not only simplify the process but also ensure that the electron dose is minimized and the region of interest is maximized. Furthermore, certain applications of these 3-dimensional reconstruction techniques require literally hundreds of images per data set and these applications for practical considerations will only be feasible given the availability of direct digital data acquisition. These control and imaging capabilities will also greatly improve the routine acquisition of high quality images. Further advantages of the new microscope arise from the advances in technology over the past 20 years. These have led to simplification of alignment procedures, improvements in beam coherence, lenses and goniometer stages and the implementation of techniques for acquiring images at low doses in order to limit beam damage. The microscope will be located in the Beckman Visualization Facility which houses a number of other related instruments (including a confocal microscope, a stereology workstation and various light microscopes) as well as supporting equipment (wet lab, microtomes, darkrooms) and excellent facilities for the digital processing and analysis of images. The location of the microscope in the multi- disciplinary environment of the Beckman Institute, which also includes part of the National Center for Supercomputing Applications, provides an excellent environment to take advantage of some of the latest technology available for the on- line processing and analysis of electron micrographs. We are strongly committed to providing access to this instrument and its associated, special facilities to the campus wide user group whose research wholly or partly depends on the application of electron microscopy. There is enthusiastic commitment to this project from both the Beckman In stitute and the University as a whole. This has been demonstrated by the contribution of 50% matching funds for the project as well as the supporting infrastructure for the equipment provided by the Beckman Institute Visualization Facility.

本提案的目的是申请资金，用于购买一台飞利浦CM- 200透射电子显微镜，该显微镜将安装在伊利诺斯大学校园贝克曼研究所的可视化设施内。 主要研究者将使用该电子显微镜进行各种研究，包括（i）神经可塑性及其与基础细胞和系统水平过程的关系（William Greenough），（ii）单分散棒-线圈嵌段共聚物的研究（塞缪尔·斯图普），（iii）核和染色体结构分析（安德鲁贝尔蒙特），（四）性别差异的组织和行为功能的大脑（Janice Juraska），和（v）运动功能障碍的遗传动物模型中小脑神经回路中的形态和功能异常的表征（Louise Abbott）除了这些核心项目外，还有来自不同学科的6个其他研究项目，该工具为这些项目提供了直接或长期的好处。 获得一份新文书有两个目的。 首先，它将为UIUC校园的生物研究提供一个中压仪器。 该地区唯一的中压仪器专门用于材料科学研究，而生物学家必须前往偏远地区进行更高电压的项目。 其次，它将取代目前位于BVF的现有18年的仪器，该仪器越来越多地受到与年龄有关的问题的困扰。 除了提供独特的成像能力较厚的标本，这种显微镜将有一些其他重要的优势，超过现有的仪器。 通过计算机接口控制仪器和使用CCD相机收集显微照片的能力将大大有助于，并且在某些情况下对于几种应用是必不可少的。 具体而言，对于涉及大量图像的收集或检查的诸如连续切片、定量体视学和单轴断层摄影的技术，可以设计半自动采集程序，其不仅简化了过程，而且确保电子剂量最小化并且感兴趣区域最大化。此外，这些三维重建技术的某些应用需要每个数据集的数百个图像，并且这些应用仅在给定直接数字数据采集的可用性的情况下才是可行的。 这些控制和成像能力也将大大改善高质量图像的常规采集。 新显微镜的进一步优势来自于过去20年的技术进步。 这些措施简化了校准程序，改进了光束相干性、透镜和测角仪载物台，并采用了在低剂量下获取图像的技术，以限制光束损害。 显微镜将位于Beckman可视化设施中，该设施容纳了许多其他相关仪器（包括共聚焦显微镜、体视学工作站和各种光学显微镜）以及支持设备（湿实验室、切片机、暗室）和用于图像数字处理和分析的优秀设施。 在贝克曼研究所的多学科环境中的显微镜的位置，其中还包括国家超级计算应用中心的一部分，提供了一个很好的环境，以利用一些最新的技术可用于在线处理和分析电子显微照片。 我们坚定地致力于提供访问该仪器及其相关的，特殊的设施，以校园范围内的用户群，其研究完全或部分依赖于电子显微镜的应用。 贝克曼研究所和整个大学对这个项目都有热情的承诺。 为该项目提供50%的配套资金以及贝克曼研究所可视化设施提供的设备支持基础设施就证明了这一点。