Enchancement and Upgrade of the DNA Sequencing Facility: Upgrade of Automated Sequencer (ABI 37 3A)/Electrophoresis Unit

DNA测序设备的增强和升级：自动测序仪（ABI 37 3A）/电泳装置的升级

• 批准号: 9318078
• 负责人: Joseph Travis
• 金额: $ 5.27万
• 依托单位: Florida State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-06-15 至 1996-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9318078&HistoricalAwards=false
• 关键词: Enchancement; Upgrade; DNA; Sequencing; Facility

We are proposing to purchase a number of items of equipment and software that will enhance the capabilities and services of our DNA Sequencing Core Facility by upgrading our Applied Biosystems 373A Electrophoresis Unit (= automated sequencer) and its associated equipment and providing the users with enhanced access to data-analysis work stations. The hardware includes a five-filter wheel (to permit sequencing with T7 DNA polymerase), a liner upgrade (to permit the option of using several variable well-to-read gel plates), vertical agarose gel plates (to facilitate analysis of 2-3 kb-fragments and random amplified polymorphic DNA), and computer equipment (to increase analytical speed and accommodate multiple users). The software includes material to facilitate sequence alignment, increase the speed of scoring fragments, and create consensus sequences. The enhancements will serve a diversity of research projects in different disciplines, from phylogenetic reconstructions (cyanobacteria from extreme environments, the major crustacean lineages) to examinations of gene expression (flagellar regeneration in Chlamydomonas, acclimatory change in muscle tissue) to the determination of reproductive patterns in natural populations (mating in livebearing fishes). The upgrade will expand the range of applications that the facility can offer and permit more rapid sample analysis, thereby enabling users from several disciplines to expand readily the scope of their work and to increase their productivity.

我们建议购买一些设备和软件，通过升级我们的应用生物系统373 A电泳单元（=自动测序仪）及其相关设备，并为用户提供更好的数据分析工作站，来提高我们的DNA测序核心设施的能力和服务。 硬件包括一个五过滤轮（允许用T7 DNA聚合酶测序），一个线性升级（允许选择使用几个可变孔来读取凝胶板），垂直琼脂糖凝胶板（以便于分析2-3 kb片段和随机扩增多态性DNA），和计算机设备（以提高分析速度和适应多个用户）。 该软件包括促进序列比对、提高片段评分速度和创建共有序列的材料。 增强将服务于不同学科的研究项目的多样性，从系统发育重建（极端环境中的蓝细菌，主要的甲壳类动物谱系）到基因表达的检查（披衣菌中的鞭毛再生，肌肉组织的适应性变化），以确定自然种群的生殖模式（在活产鱼类中交配）。 这一升级将扩大该设施可提供的应用范围，并允许更迅速地进行样品分析，从而使若干学科的用户能够迅速扩大其工作范围，提高其生产力。