Biotechnolgy Facility for Analytical Ultracentrifugation

分析型超速离心机将主要参与先进分析型超速离心法的开发和应用，硬件和软件。光学和数据采集系统的改进将应用于新的XL-A离心机的吸收光学和干涉光学。这些创新将直接应用于增加这种新的第二代离心机的范围和功率。应用将主要涉及大分子相互作用的平衡沉淀研究，特别强调异缔合。这还将涉及数据分析软件的开发以及优化这类研究的研究战略。将设计和应用新的技术发展，以提高速度沉积测量的准确性和效率，这项技术在上一笔赠款中没有得到高度优先考虑。在过去三年半的时间里，科学界已经获得了这些资源。该机构已经参与或仍在参与31个研究项目(已完成17个)，涉及100多个大分子样品和实验条件的组合。在今后五年中，与学术界和工业界研究人员的这种合作以及发表这类研究的结果将继续是该机制的中心重点。该设施还通过改进仪器和计算方法，帮助研究人员利用他们自己实验室中新开发的技术。通过在国家会议和大会上的发言，以及在该设施为调查人员、学生和技术人员举办的讲习班，促进了这一转移。这项培训，连同分发软件和硬件的原理图和施工图，将构成该基金技术转让任务的核心。

The Analytical Ultracentrifugation facility will be heavily involved in the development and the application of methodology, hardware and software for advanced analytical ultracentrifugation. Optical and data acquisition system improvements will be applied to both absorption and interference optics of the new XL-A centrifuge. These innovations will have direct application to increasing the scope and power of this new second-generation centrifuge. Applications will deal largely with equilibrium sedimentation studies of macromolecular interactions with particular emphasis on hetero-association. This will also involve the development of software for data analysis as well as research strategies to optimize such studies. New technical developments will be engineered and applied to increase the accuracy and efficiency of velocity sedimentation measurements, a technique which did not receive high priority under the previous grant. These resources have been made available to the scientific community over the past three and a half years. The Facility has participated in or is still involved with 31 research projects (17 completed) involving over 100 combinations of macromolecular samples and experimental conditions. During the next five years this collaboration with academic and industrial researchers and the publication of the results of such research will continue to be the central focus of the Facility. The Facility also assists researchers to make use of the newly developed technology in their own laboratories by upgrading their instrumentation and computational methods. This transfer is promoted by presentations at national meetings and conferences, and by workshops held at the Facility for investigators, students, and technicians. This training, along with the distribution of software and of the schematics and construction diagrams for hardware, will constitute the heart of the technology-transfer mission of the Facility.