Biotechnolgy Facility for Analytical Ultracentrifugation

分析超浓缩设施将大量参与先进分析超浓缩方法、硬件和软件的开发和应用。 光学和数据采集系统的改进将适用于新的XL-A离心机的吸收和干涉光学。 这些创新将直接应用于增加这种新的第二代离心机的范围和功率。 应用程序将主要涉及大分子相互作用的平衡沉降研究，特别强调杂缔合。 这还将涉及开发数据分析软件以及优化此类研究的研究战略。 新的技术发展将被设计和应用，以提高速度沉积测量的准确性和效率，这项技术在以前的赠款中没有得到高度优先考虑。 在过去三年半的时间里，这些资源已提供给科学界。 该设施参与或仍在参与31个研究项目（17个已完成），涉及100多种高分子样品和实验条件的组合。 在今后五年中，与学术界和工业界研究人员的这种合作以及发表这种研究的结果将继续是该基金的中心重点。 该设施还协助研究人员在自己的实验室中利用新开发的技术，升级他们的仪器和计算方法。 在国家会议和大会上的介绍以及在该设施为调查人员、学生和技术人员举办的讲习班促进了这种转让。 这项培训，连同分发软件和硬件的原理图和结构图，沿着将构成该设施技术转让使命的核心。

The Analytical Ultracentrifugation facility will be heavily involved in the development and the application of methodology, hardware and software for advanced analytical ultracentrifugation. Optical and data acquisition system improvements will be applied to both absorption and interference optics of the new XL-A centrifuge. These innovations will have direct application to increasing the scope and power of this new second-generation centrifuge. Applications will deal largely with equilibrium sedimentation studies of macromolecular interactions with particular emphasis on hetero-association. This will also involve the development of software for data analysis as well as research strategies to optimize such studies. New technical developments will be engineered and applied to increase the accuracy and efficiency of velocity sedimentation measurements, a technique which did not receive high priority under the previous grant. These resources have been made available to the scientific community over the past three and a half years. The Facility has participated in or is still involved with 31 research projects (17 completed) involving over 100 combinations of macromolecular samples and experimental conditions. During the next five years this collaboration with academic and industrial researchers and the publication of the results of such research will continue to be the central focus of the Facility. The Facility also assists researchers to make use of the newly developed technology in their own laboratories by upgrading their instrumentation and computational methods. This transfer is promoted by presentations at national meetings and conferences, and by workshops held at the Facility for investigators, students, and technicians. This training, along with the distribution of software and of the schematics and construction diagrams for hardware, will constitute the heart of the technology-transfer mission of the Facility.