Molecular Population Genetics of Inversion Polymorphism

9318381染色体重排是动植物基因组的一个重要特征，也是癌症的一个重要原因。然而，到目前为止，人们对这种安排的断点特征知之甚少。这个项目将以黑腹果蝇作为模式生物，研究与自然种群中发现的三个倒位的染色体断裂点相关的DNA序列特征。这也将检验黑斑潜蝇和拟黑斑潜蝇之间的一个固定的倒置差异。这项拟议的研究将使用一种已建立的显微切割-聚合酶链式反应方法来有效恢复跨越断点的基因组克隆。随后对这些克隆和倒置和标准排列的总体样本的分析将允许对一些长期感兴趣的假设进行评估。这些包括确定重排是否是每个独特的突变事件，并导致基因突变。它还将允许我们确定一些反转是否旧。总体而言，我们的研究将为倒位多态的突变起源、表型后果和群体遗传学提供新的信息。果蝇的种类。果蝇作为分子变化的模型正在迅速发展，特别是在单基因变异方面。这项研究将为我们更全面地理解染色体重排做出独特而重要的贡献。P d z__！u！F_(Times New Roman符号&amp；Arial“h Z E c e c e 7水晶黑剪力水晶黑剪力

9318381 Eanes Chromosomal rearrangements are a significant feature of animal and plant genomes, and are a significant cause of cancers. However, to date little is known about the features of the breakpoints of such arrangements. Using Drosophila melangaster as a model organism, this project will examine DNA sequence features associated with the chromosomal breakpoints of three inversions found to be polymorphic in natural populations. This will also examine the one fixed inversion difference between D. melangaster and D. simulans. The proposed investigation will use an established microdissection-polymerase chain reaction approach to efficiently recover genomic clones spanning breakpoints. The subsequent analysis of these clones and population samples of inverted and standard arrangements will permit evaluation of a number of hypotheses of long standing interest. These include determining whether rearrangements are each unique mutational events, and cause mutations in genes. It will also allow us to determine if some inversions are old. Overall, our study will contribute novel information specific to the mutational origin, phenotypic consequences, and population genetics of inversion polymorphism. The fruit fly species. Drosophila melangaster, is growing rapidly as a model of molecular change particularly with respect to variation in single genes. This study will add a unique and significant contribution to our understanding chromosomal rearrangements in general. p d z _ _ !u ! F _ _ ( Times New Roman Symbol & Arial " h Z E c e c e 7 Crystal Blackshear Crystal Blackshear