Molecular Population Genetics of Inversion Polymorphism

小行星9318381 染色体重排是动物和植物基因组的重要特征，并且是癌症的重要原因。 然而，迄今为止，人们对这种安排的断点的特征知之甚少。 以黑腹果蝇为模式生物，本计画将检视自然族群中三种具多态性倒位染色体断裂点的DNA序列特徴。 这也将检查D之间的一个固定反转差异。melangaster和D. simulans。 拟议的调查将使用一个既定的显微切割聚合酶链反应的方法，以有效地恢复跨越断点的基因组克隆。 随后的分析，这些克隆和人口样本的倒置和标准的安排将允许评估的一些假说的长期利益。 这些包括确定重排是否是每个独特的突变事件，并导致基因突变。 它还将允许我们确定一些反演是否是旧的。 总的来说，我们的研究将有助于新的信息，具体的突变起源，表型的后果，和群体遗传学的倒位多态性。 果蝇的种类黑腹果蝇作为分子变化的模型，特别是在单基因变异方面，正在迅速发展。 这项研究将增加一个独特的和显着的贡献，我们了解染色体重排一般。 p D z _ _ ! u ! F _ _ ( Times New Roman 符号Arial " H Z E c e c e 7 水晶黑剪水晶黑剪

9318381 Eanes Chromosomal rearrangements are a significant feature of animal and plant genomes, and are a significant cause of cancers. However, to date little is known about the features of the breakpoints of such arrangements. Using Drosophila melangaster as a model organism, this project will examine DNA sequence features associated with the chromosomal breakpoints of three inversions found to be polymorphic in natural populations. This will also examine the one fixed inversion difference between D. melangaster and D. simulans. The proposed investigation will use an established microdissection-polymerase chain reaction approach to efficiently recover genomic clones spanning breakpoints. The subsequent analysis of these clones and population samples of inverted and standard arrangements will permit evaluation of a number of hypotheses of long standing interest. These include determining whether rearrangements are each unique mutational events, and cause mutations in genes. It will also allow us to determine if some inversions are old. Overall, our study will contribute novel information specific to the mutational origin, phenotypic consequences, and population genetics of inversion polymorphism. The fruit fly species. Drosophila melangaster, is growing rapidly as a model of molecular change particularly with respect to variation in single genes. This study will add a unique and significant contribution to our understanding chromosomal rearrangements in general. p d z _ _ !u ! F _ _ ( Times New Roman Symbol & Arial " h Z E c e c e 7 Crystal Blackshear Crystal Blackshear