Molecular Population Genetics of Inversion Polymorphism

9318381 Eanes染色体重排是动物和植物基因组的重要特征，也是癌症的重要原因。然而，迄今为止，人们对这种安排的断点的特征所知甚少。以果蝇为模型生物，本项目将研究与自然种群中发现的三种多态倒位染色体断点相关的DNA序列特征。这也将检验D. melangaster和D. simulans之间一个固定的反演差异。拟议的调查将使用已建立的微解剖-聚合酶链反应方法来有效地恢复跨越断点的基因组克隆。对这些反向排列和标准排列的克隆和总体样本的后续分析将允许对一些长期存在的假设进行评估。这些包括确定重排是否是每个独特的突变事件，并导致基因突变。它还将使我们能够确定一些逆序是否已经过时。总的来说，我们的研究将为反转多态性的突变起源、表型后果和群体遗传学提供新的信息。果蝇种类。黑果蝇，作为分子变化的一个模型正在迅速发展，特别是在单基因变异方面。这项研究将为我们对染色体重排的理解做出独特而重要的贡献。P d z __!你!F _ (Times New Roman Symbol & & Arial " h Z E c E c E 7 Crystal Blackshear水晶Blackshear

9318381 Eanes Chromosomal rearrangements are a significant feature of animal and plant genomes, and are a significant cause of cancers. However, to date little is known about the features of the breakpoints of such arrangements. Using Drosophila melangaster as a model organism, this project will examine DNA sequence features associated with the chromosomal breakpoints of three inversions found to be polymorphic in natural populations. This will also examine the one fixed inversion difference between D. melangaster and D. simulans. The proposed investigation will use an established microdissection-polymerase chain reaction approach to efficiently recover genomic clones spanning breakpoints. The subsequent analysis of these clones and population samples of inverted and standard arrangements will permit evaluation of a number of hypotheses of long standing interest. These include determining whether rearrangements are each unique mutational events, and cause mutations in genes. It will also allow us to determine if some inversions are old. Overall, our study will contribute novel information specific to the mutational origin, phenotypic consequences, and population genetics of inversion polymorphism. The fruit fly species. Drosophila melangaster, is growing rapidly as a model of molecular change particularly with respect to variation in single genes. This study will add a unique and significant contribution to our understanding chromosomal rearrangements in general. p d z _ _ !u ! F _ _ ( Times New Roman Symbol & Arial " h Z E c e c e 7 Crystal Blackshear Crystal Blackshear