Molecular Population Genetics of a Phosphoglucose Isomerase Polymorphism: Testing Hypotheses of Balancing Selection and Trans-specific Polymorphism

磷酸葡萄糖异构酶多态性的分子群体遗传学：检验平衡选择和反式特异性多态性的假设

• 批准号: 9318472
• 负责人: Richard Harrison
• 金额: $ 5万
• 依托单位: Cornell Univ - State: AWDS MADE PRIOR MAY 2010
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-07-01 至 1995-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9318472&HistoricalAwards=false
• 关键词: Molecular; Population; Genetics; Phosphoglucose; Isomerase

HARRISON 9318472 National Populations (including human populations) often contain large amounts variation at genes coding for soluble enzymes. The functional significance of this enzyme variation remains a matter of considerable debate. Do enzyme variants in natural populations differ in their functional properties? If so, what forces maintain variation within populations. This project focuses on using DNA sequencing of different allelic forms of the enzyme phosphoglucose isomerase (PGI) to determine what is acting to maintain enzyme variation within species of insects. PGI occupies a central position in carbohydrate metabolism. Catalyzing the interconversion of glucose-6-phosphate and fructose-6-phosphate. The first goal of this research is to characterize the structure of the Pgi gene in two species of crickets, which are both variable for PGI and which have the "same" array of allelic variants (allozymes). Representatives of each of the allelic variants in the two species will then be sequenced. Of particular interest is whether some or all of the amino acid differences that distinguish the allozyme classes are identical in the two species and whether observed amino acid differences are likely maintained in populations by balancing selection. The results will shed light on the general question of the significance of genetic variation for soluble enzymes. Resolution of this issue has implications for understanding the significance of genetic variation in human populations and for questions related to long-term conservation of genetic resources.

哈里森9318472 国家群体（包括人类群体）通常在编码可溶性酶的基因上含有大量变异。 这种酶变异的功能意义仍然是一个相当有争议的问题。 自然群体中的酶变体在功能特性上是否不同？ 如果是这样，是什么力量维持着种群内的变异？ 该项目的重点是使用磷酸葡萄糖异构酶（PGI）的不同等位基因形式的DNA测序，以确定是什么在昆虫物种内保持酶的变异。 PGI在碳水化合物代谢中占据中心地位。 催化葡萄糖-6-磷酸和果糖-6-磷酸的相互转化。 本研究的第一个目标是描述两种蟋蟀的PGI基因的结构，这两种蟋蟀都是PGI可变的，并且具有“相同”的等位基因变体（等位酶）阵列。 然后将对两个物种中每个等位基因变体的代表进行测序。 特别令人感兴趣的是区分等位酶类别的一些或所有氨基酸差异在两个物种中是否相同，以及观察到的氨基酸差异是否可能通过平衡选择在种群中保持。 这些结果将阐明可溶性酶遗传变异的意义这一普遍问题。 这一问题的解决对理解人类群体遗传变异的重要性以及与遗传资源的长期保护有关的问题具有重要意义。