Solvatochromic Studies of Solvated Stationary Phases

溶剂化固定相的溶剂变色研究

• 批准号: 9318484
• 负责人: Sarah Rutan
• 金额: $ 14万
• 依托单位: Virginia Commonwealth University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-03-01 至 1996-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9318484&HistoricalAwards=false
• 关键词: Solvatochromic; Studies; Solvated; Stationary; Phases

9318484 Hawkridge This award is made in the Analytical and Surface Chemistry Program in support of the research of Dr. Sarah Rutan. The research is aimed at elucidating the role of the solvated stationary phase in reversed phase liquid chromatography. This will be achieved through the use of indicators whose UV-visible absorbance maxima are dependent on their molecular environments. Solvatochromic parameters describing the dipolarity/polarizability, hydrogen bond acidity and hydrogen bond basicity will be determined. Spectra of various indicators in mixed aqueous/organic supensions of stationary phase particles which have been derivatized with alkyl chains will be obtained using diffuse reflectance spectroscopy. Interferences from dye molecules that remain in the bulk mobile phase will be eliminated using adaptive filtering techniques. In a related study, factor analysis methods will be used to aid in the development of methods for the prediction of infinite dilution activity coefficients and heats of solution, based on these solvatochromic parameters. The combination of improved modeling techniques and the development of parameters describing the chemistry of the chromatographic phases should ultimately allow the researcher to predict retention characteristics for solutes in reversed phase liquid chromatography. This capability will be of significant benefit across a broad range of industrial and medically related separations and may also prove valuable to the characterization of other interfacial systems.

9318484 Hawkridge 该奖项是在分析和表面化学项目中颁发的，旨在支持 Sarah Rutan 博士的研究。该研究旨在阐明溶剂化固定相在反相液相色谱中的作用。这将通过使用其紫外-可见吸收最大值取决于其分子环境的指示剂来实现。将确定描述偶极性/极化性、氢键酸性和氢键碱性的溶剂致变色参数。使用漫反射光谱获得已用烷基链衍生的固定相颗粒的混合水/有机悬浮液中各种指示剂的光谱。使用自适应过滤技术可以消除残留在本体流动相中的染料分子的干扰。 在相关研究中，因子分析方法将用于帮助开发基于这些溶剂化显色参数预测无限稀释活度系数和溶解热的方法。 改进的建模技术和描述色谱相化学参数的开发相结合，最终将使研究人员能够预测反相液相色谱中溶质的保留特性。这种能力将为广泛的工业和医学相关分离带来显着的好处，并且对于其他界面系统的表征也可能有价值。