Solvatochromic Studies of Solvated Stationary Phases

9318484 Hawkridge该奖项是在分析和表面化学计划，以支持博士的研究。本研究旨在阐明溶剂化固定相在反相液相色谱中的作用。这将通过使用其紫外-可见吸收最大值取决于其分子环境的指示剂来实现。将确定描述偶极性/极化率、氢键酸度和氢键碱度的溶剂化变色参数。用漫反射光谱法测定了烷基链衍生化的固定相颗粒的混合水/有机悬浮液中各种指示剂的光谱。使用自适应滤波技术消除残留在本体移动的相中的染料分子的干扰。 在一项相关的研究中，将使用因子分析方法来帮助制定根据这些溶致变色参数预测无限稀释活度系数和溶解热的方法。 改进的建模技术和描述色谱相的化学参数的发展相结合，最终应允许研究人员预测在反相液相色谱中溶质的保留特性。这种能力将在广泛的工业和医学相关的分离，也可能证明其他界面系统的表征有价值的显着好处。

9318484 Hawkridge This award is made in the Analytical and Surface Chemistry Program in support of the research of Dr. Sarah Rutan. The research is aimed at elucidating the role of the solvated stationary phase in reversed phase liquid chromatography. This will be achieved through the use of indicators whose UV-visible absorbance maxima are dependent on their molecular environments. Solvatochromic parameters describing the dipolarity/polarizability, hydrogen bond acidity and hydrogen bond basicity will be determined. Spectra of various indicators in mixed aqueous/organic supensions of stationary phase particles which have been derivatized with alkyl chains will be obtained using diffuse reflectance spectroscopy. Interferences from dye molecules that remain in the bulk mobile phase will be eliminated using adaptive filtering techniques. In a related study, factor analysis methods will be used to aid in the development of methods for the prediction of infinite dilution activity coefficients and heats of solution, based on these solvatochromic parameters. The combination of improved modeling techniques and the development of parameters describing the chemistry of the chromatographic phases should ultimately allow the researcher to predict retention characteristics for solutes in reversed phase liquid chromatography. This capability will be of significant benefit across a broad range of industrial and medically related separations and may also prove valuable to the characterization of other interfacial systems.