Collaborative Research: Microbial and Geochemical Controls on Metal Cycling in Lake Vanda

9319044绿湖万达是一个被冰覆盖的永久分层的湖。水柱深69米，呈现了广泛的地球化学和微生物环境。该湖1200年的层化历史和水柱的稳定性使其成为探索化学过程与微生物多样性和活动之间关系的模式系统。特别令人感兴趣的是连续的还原和氧化带与这些带的微生物和化学特征之间的关系。格林博士和他的同事计划使用一系列测量方法对化学和微生物群落结构进行第一次同步研究，包括通过显微镜和核酸提取和鉴定来确定微生物生物量和多样性。这项工作的核心是锰还原生物在万达水柱的金属循环中所起的作用。微生物介导的锰循环的能力将通过确定锰还原剂的丰度和锰还原所涉及的机制来评估。此外，计划沿着玛瑙河27公里长的河道对金属运输机制进行详细研究，奥尼斯河是该湖唯一的流入河流。将确定金属负荷量和在湖中的停留时间。***

9319044 Green Lake Vanda is an ice-covered, permanently stratified lake. The water column, 69-meter deep, presents a wide range of geochemical and microbiological environments. The lake's 1200-year stratification history and its water column stability make it a model system for exploring the relationship between chemical processes and microbial diversity and activity. Of special interest is the relationship between successive reductive and oxidative zones and the microbial and chemical characteristics of those zones. Dr. Green and colleague plan the first simultaneous study of chemical and microbial community structure using a combination of measurements, including determinations of microbial biomass and diversity as estimated by microscopy and nucleic acid extraction and identification. Central to this work is the role played by manganese-reducing organisms in metal cycling in the Vanda water column. The capacity for microbially mediated manganese cycling will be assessed by determining the abundance of manganese reducers and the mechanisms involved in Mn reduction. In addition, a detailed study of metal transport mechanisms is planned along the 27-km course of the Onyx River, the lake's sole inflow. Determination of metal loadings and residences times in the lake will be made. ***