Separation of Complex Materials by Field-Flow Fractionation

通过场流分级分离复杂材料

• 批准号: 9322472
• 负责人: Joel Harris
• 金额: $ 56.47万
• 依托单位: University of Utah
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-05-01 至 1998-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9322472&HistoricalAwards=false
• 关键词: Separation; Complex; Materials; Field; Flow

This research, supported by the Analytical and Surface Chemistry Program, is focused on the development of Field Flow Fractionation (FFF), a technique based on the use of orthogonal force fields to separate large molecules and small particles. The analyte mixture is pumped into a rectangular channel, and a force is applied 90 degrees relative to the direction of flow. In particular, this grant will explore the use of thermal gradients and gravitational forces to effect the separation. In addition, analytical split flow thin cell (SPLITT) fractionation, a method where the separation occurs along the channel width, and where splitters are used at one or both ends of the channel, is investigated to determine it's potential for analytical separations. Here, the separation is based on hydrodynamic lift forces, which allows characterization of species based on diffusion coefficients. These techniques can be used to characterize a variety of biological and synthetic materials. This grant in the Analytical and Surface Chemistry Program will support research into improved methods for the separation of large molecules and small particles using field flow fractionation. This is a procedure invented by Professor Giddings in which crossed force fields are used to separate small (micron-sized) particles or molecules based on differences in shape, density, and electrical characteristics. This methodology will have wide-ranging applications in the fields of biotechnology and materials science.

这项研究得到了分析和表面化学项目的支持， 专注于场流分级（FFF）的发展， 技术的基础上使用正交力场分离大 分子和小颗粒。 将分析物混合物泵入 矩形通道，并且相对于矩形通道施加90度的力。 流动方向。 特别是，这笔赠款将探索使用 热梯度和重力来实现分离。 在 此外，分析分流薄细胞（SPLITT）分馏， 其中分离沿着沟道宽度发生，并且其中 在通道的一端或两端使用分离器，研究 确定它用于分析分离的潜力。 这里 分离是基于流体动力学升力， 基于扩散系数的物种表征。 这些 技术可用于表征多种生物学和 合成材料。 分析和表面化学计划的这笔赠款将支持 研究改进的分离大分子的方法， 使用场流分离的小颗粒。 这个程序 由吉丁斯教授发明，交叉力场被用来 基于差异分离小（微米级）颗粒或分子 形状、密度和电特性。 这种方法将 在生物技术领域具有广泛的应用， 材料科学