Roles of Structural MAPs in Cytoplasmic Organization (Biological Science)

结构 MAP 在细胞质组织中的作用（生物科学）

• 批准号: 9350145
• 负责人: Joanna Olmsted
• 金额: $ 9.48万
• 依托单位: University of Colorado at Boulder
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-07-01 至 1995-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9350145&HistoricalAwards=false
• 关键词: Roles; Structural; MAPs; Cytoplasmic; Organization

Dr. Olmsted's research will primarily involve investigating the role of structural microtubule-associated proteins (MAPs) in microtubule organization and function. Approaches that combine immunological and high resolution light and electron microscopy will be used to determine the distribution of MAP 4 in male gametic cells and differentiating myoblasts. Genetically engineered constructs transfected into cells will be examined for their effects on the organization of endogenous microtubule arrays and other cellular structures. Phenotypes generated by antisense experiments will be studied to see how absence of MAP 4 affects miotic structures. Electron microscopic analyses of microinjected MAP 2 interactions with the Golgi will also be carried out. Pilot experiments to identify a MAP 4 homolog in Schizosaccharomyces pombe will also be undertaken. Interactive activities at the host institution include: presenting a seminar on the choice of science as a career and the attraction and retention of women into science; a graduate seminar on cytoskeletal-organelle interactions; guest lectures in introductory and cell biology courses; and participation in weekly laboratory meetings and seminars. This project furthers VPW program objectives which are (1) to provide opportunities for women to advance their careers in engineering and in the disciplines of science supported by NSF and (2) providing greater visibility for women scientists and engineers employed in industry, government, and academic institutions. By encouraging the participation of women in science, it is a valuable investment in the Nation's future scientific vitality.

奥姆斯特德博士的研究将主要涉及调查 结构微管相关蛋白（MAP）在 微管的组织和功能。 联合收割机 免疫学和高分辨率光学和电子显微镜 将用于确定MAP 4在雄性配子体中的分布 细胞和分化的成肌细胞。 基因工程 将检查转染到细胞中的构建体的 对内源性微管阵列组织的影响， 其他细胞结构。 反义核酸产生的表型 将研究实验，看看缺乏MAP 4如何影响 缩瞳结构。 显微注射的电镜分析 还将进行MAP 2与高尔基体的相互作用。 试点 在裂殖酵母中鉴定MAP 4同源物的实验 还将进行pombe。 主办机构的互动活动包括： 一个关于选择科学作为职业和吸引力的研讨会 研究生研讨会， 细胞器-细胞器相互作用;客座讲座介绍 和细胞生物学课程;并参加每周的实验室 会议和研讨会。 本项目进一步促进了VPW计划的目标，即（1） 为妇女提供机会， 工程和科学的学科由NSF支持， (2)提高女科学家和女工程师的知名度 受雇于工业、政府和学术机构。 通过 鼓励妇女参与科学，这是一个宝贵的 投资于国家未来的科学活力。