A Confocal Microscope for Cell and Developmental Biology Research

用于细胞和发育生物学研究的共焦显微镜

• 批准号: 9512886
• 负责人: Joanna Olmsted
• 金额: $ 11.2万
• 依托单位: University of Rochester
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9512886&HistoricalAwards=false
• 关键词: Confocal; Microscope; Cell; Developmental; Biology

This application requests funds for the purchase of a Bio-Rad confocal system integrated with a Nikon microscope. The seven major users of this facility are junior and senior faculty in the Department of Biology at the University of Rochester, and all are funded from national granting agencies (NSF, NIH, USDA). The current programs of the users involve carrying out studies on fate specification, cell-cell interactions and morphogenesis in developing organisms (Drosophila, sea urchins, Arabidopsis); the role of cytoskeletal elements in cellular and organismal functions; the mechanisms of nuclear transport; and regulation of genome packaging. These projects are unified in using molecular, genetic and/or biochemical techniques to ask how specific gene products influence cellular and developmental processes. In order to carry out quantitation, dynamic analysis and co-localization studies, all of these projects now require a confocal microscope system which is: 1) reliable and easily used by a number of individuals; 2) has multiple types of optics; 3) has flexibility in the excitation and emission wavelengths; and 4) has excellent resolution in the Z and X/Y axes. Image analysis software that allows further quantification and manipulation of the confocal data is also requested. As members of the Department of Biology, all of the users are also involved in education of undergraduate and graduate students in both formal and informal settings. In the context of each of the research projects described, the requested instrumentation will be used by graduate students and postdoctorals in training. The instrumentation will also be employed for demonstrations in undergraduate courses and outreach programs, and for hands-on experiences- in advanced laboratory courses and independent undergraduate research projects.

该申请请求资金购买与尼康显微镜集成的 Bio-Rad 共焦系统。该设施的七个主要用户是罗切斯特大学生物系的初级和高级教师，全部由国家资助机构（NSF、NIH、USDA）资助。用户当前的项目涉及对发育中生物体（果蝇、海胆、拟南芥）的命运规范、细胞间相互作用和形态发生进行研究；细胞骨架元素在细胞和有机体功能中的作用；核运输机制；和基因组包装的调控。这些项目统一使用分子、遗传和/或生化技术来探究特定基因产物如何影响细胞和发育过程。为了进行定量、动态分析和共定位研究，所有这些项目现在都需要共焦显微镜系统，该系统具有以下特点：1）可靠且易于多人使用； 2）具有多种类型的光学器件； 3）激发和发射波长具有灵活性； 4) 在Z和X/Y轴上具有出色的分辨率。还需要能够进一步量化和操作共焦数据的图像分析软件。 作为生物系的成员，所有用户还参与正式和非正式环境中本科生和研究生的教育。在所描述的每个研究项目的背景下，研究生和博士后将在培训中使用所需的仪器。该仪器还将用于本科课程和推广项目的演示，以及高级实验室课程和独立本科研究项目的实践经验。