Investigative Instructional Improvements in Molecular Biology and Organellar DNA Analysis

分子生物学和细胞器 DNA 分析的研究性教学改进

• 批准号: 9351802
• 负责人: Joby Chesnick
• 金额: $ 2.69万
• 依托单位: Lafayette College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-06-01 至 1995-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9351802&HistoricalAwards=false
• 关键词: Investigative; Instructional; Improvements; Molecular; Biology

The aim of this project is to advance student training and experience in investigative molecular biology. Specifically, the requested equipment, consisting of a high speed centrifuge and rotors, thermocycler, electroporator, nucleotide sequencing and agarose gel electrophoresis equipment and related accessories, will support a new research-intensive advanced investigative molecular biology course in which students will characterize algal mitochondrial and chloroplast genome architecture and gene structure. The proposed equipment will be used to increase the sophistication of strategies used for nucleic acid analysis including the isolation of purified organellar DNA, DNA library construction, genome mapping, and nucleotide sequence analysis of selected genes. Fundamental principles of DNA structure and function and basic techniques are presented in existing intorductory courses. Investigative Molecular Biology will offer opportunities that (1) provide for advanced study of DNA organization and function; (2) serve as an avenue for student participation in investigative research; and (3) for biology majors, function to train and focus those students who anticipate entering into molecular biology related honors research projects.

该项目的目的是促进学生在分子生物学研究方面的训练和经验。具体来说，所要求的设备包括高速离心机和转子、热循环仪、电孔仪、核苷酸测序和琼脂糖凝胶电泳设备及相关配件。将支持一门新的研究密集型高级研究性分子生物学课程，学生将在该课程中描述藻类线粒体和叶绿体基因组结构和基因结构。该设备将用于提高用于核酸分析的策略的复杂性，包括纯化细胞器DNA的分离，DNA文库的构建，基因组定位和选定基因的核苷酸序列分析。DNA结构和功能的基本原理和基本技术在现有的入门课程中介绍。研究分子生物学将提供机会：(1)提供DNA组织和功能的高级研究；(2)作为学生参与调查研究的途径；(3)对于生物学专业的学生，其功能是培养和关注那些期望进入分子生物学相关荣誉研究项目的学生。