NSF Young Investigator/Electrochemical Microsensors and Microcolumn Separation: Analysis of Peptide Neuro- Transmitters and Hormones

NSF 青年研究员/电化学微传感器和微柱分离：肽神经递质和激素的分析

• 批准号: 9357411
• 负责人: Robert Kennedy
• 金额: $ 31.25万
• 依托单位: University of Florida
• 依托单位国家: 美国
• 项目类别: Continuing grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-07-01 至 1997-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9357411&HistoricalAwards=false
• 关键词: NSF; Young; Investigator; Electrochemical; Microsensors

This National Science Foundation Young Investigator project is in the general area of analytical and surface chemistry and in the subfield of bioanalytical chemistry. During the five-year tenure of this award, Professor Kennedy and his students will pursue the development of electrochemical microsensors and microcolumn separations, such as capillary chromatography and capillary electrophoresis, for the investigation of peptide neurotransmitters and hormones. A major goal of the research is the detection and continuous monitoring of zeptomole quantities of peptides at single cells and in vivo with millisecond time resolution and submicron spatial resolution. %%% The successful attainment of the goals of this project should enable the broad advancement of bioanalytical chemistry, in particular the analysis of minute quantities of neuropeptides and hormones. The proposed methods, including microscale sequencing and microcolumn separations, will allow the detection of peptides in specific regions of the brain or in single cells.

这个国家科学基金会青年研究员项目是在分析和表面化学的一般领域，以及生物分析化学的子领域。在该奖项的五年任期内，肯尼迪教授和他的学生将致力于电化学微传感器和微柱分离的开发，如毛细管色谱和毛细管电泳法，用于多肽神经递质和激素的研究。这项研究的一个主要目标是以毫秒级的时间分辨率和亚微米级的空间分辨率检测和连续监测单细胞和体内的齐托莫尔多肽的量。这个项目的目标的成功实现应该能够使生物分析化学，特别是微量神经肽和激素的分析得到广泛的发展。拟议的方法，包括微型测序和微柱分离，将允许在大脑的特定区域或单个细胞中检测多肽。