NSF Young Investigator/Electrochemical Microsensors and Microcolumn Separation: Analysis of Peptide Neuro- Transmitters and Hormones

这个国家科学基金青年研究员项目是在分析和表面化学的一般领域和生物分析化学的子领域。在该奖项的五年任期内，Kennedy教授和他的学生将继续发展电化学微传感器和微柱分离，如毛细管色谱和毛细管电泳，用于肽神经递质和激素的研究。该研究的主要目标是在单细胞和体内以毫秒时间分辨率和亚微米空间分辨率检测和连续监测肽的量。该项目目标的成功实现将使生物分析化学，特别是微量神经肽和激素的分析取得广泛进展。所提出的方法，包括微尺度测序和微柱分离，将允许检测大脑特定区域或单个细胞中的肽。

This National Science Foundation Young Investigator project is in the general area of analytical and surface chemistry and in the subfield of bioanalytical chemistry. During the five-year tenure of this award, Professor Kennedy and his students will pursue the development of electrochemical microsensors and microcolumn separations, such as capillary chromatography and capillary electrophoresis, for the investigation of peptide neurotransmitters and hormones. A major goal of the research is the detection and continuous monitoring of zeptomole quantities of peptides at single cells and in vivo with millisecond time resolution and submicron spatial resolution. %%% The successful attainment of the goals of this project should enable the broad advancement of bioanalytical chemistry, in particular the analysis of minute quantities of neuropeptides and hormones. The proposed methods, including microscale sequencing and microcolumn separations, will allow the detection of peptides in specific regions of the brain or in single cells.