Chemical Monitoring Using Capillary Separations

这项工作由佛罗里达大学助理教授罗伯特·T·肯尼迪指导，主要研究与中枢神经系统神经传递过程相关的化学物质的定性和定量测量问题。由化学司的分析和表面化学方案提供支持。与毛细管分离技术相结合的小型化采样系统将被开发出来，以实现高速活体测量的长期目标。微透析法和推挽式灌注法都将作为取样系统进行研究。在线化学衍生化将产生适合于激光诱导荧光检测或电化学检测的产品。后者利用缩二脲反应与多肽类神经递质形成铜(II)络合物。为了提高灵敏度，将使用双电极检测器布置，其中络合物在阳极和阴极之间来回循环。了解神经系统功能的化学基础将对治疗神经系统疾病大有裨益。为了实现这一点，有必要识别和量化所涉及的化学物质，并能够在时间和空间上跟踪它们的变化。该项目的主要目标是为此类调查中遇到的小体积、低浓度、大量不同化学品的存在和反应时间短所带来的测量问题提供解决办法。

This work, directed by Assistant Professor Robert T. Kennedy of the University of Florida, is centered on problems of qualitative and quantitative measurement of chemicals associated with neurotransmission processes in the central nervous system. Support is provided by the Analytical and Surface Chemistry Program in the Chemistry Division. Miniaturized sampling systems coupled to capillary separation techniques will be developed with the long range goal of high speed in vivo measurements. Both microdialysis and push-pull perfusion will be investigated as sampling systems. On-line chemical derivatization will yield products suitable for laser induced fluorescence detection or electrochemical detection. The latter uses the biuret reaction to form Cu(II) complexes with peptide neurotransmitters. To enhance sensitivity, a dual electrode detector arrangment will be used in which the complex recycles back and forth between the anode and cathode. An understanding of the chemical basis of the functioning of the nervous system would be of great benefit in the treatment of neurological diseases. To achieve this, it is necessary to identify and quantify the chemicals involved and to be able to follow their changes temporily and spatially. The major objective of this project is to provide solutions to the measurement problems presented by the small volumes, the low concentrations, the presence of a large number of different chemicals and the short response time that are encountered in such investigations.