Viral Amplification of Recombinant RNA In Transgenic Plants

转基因植物中重组 RNA 的病毒扩增

• 批准号: 9402103
• 负责人: Wayne Fitzmaurice
• 金额: $ 30万
• 依托单位: Biosource Technologies Inc
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-10-01 至 1996-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9402103&HistoricalAwards=false
• 关键词: Viral; Amplification; Recombinant; RNA; Transgenic

9402103 Abstract Turpen The interaction of plants and their viruses present unique opportunities for the production of complex molecules. Tobacco Mosaic Virus (TMV) is the type member of the tobamovirus group and is one of the best understood viruses in all aspects of their biology. Using existing technology for the construction of recombinant RNA viruses and transgenic hosts, we propose to use viral RNA amplification for the synthesis of defined products in plants. In Phase II, we will determine the necessary and optimal configuration of episomal sequences to achieve the goal of controlled and biologically contained, high level gene expression in whole plants. %%% The most highly expressed genes in plants are found encoded in plant RNA viral genomes. In order to develop a plant virus as a gene vector, we will design constructs which retain the ability to generate infectious recombinants and, as such, will no longer be limited in their use as gene vectors. Thus, the production of complex molecules on a commercial basis will be greatly optimized. ***

9402103摘要Turpen植物和它们的病毒的相互作用为复杂分子的产生提供了独特的机会。烟草花叶病毒（TMV）是烟草花叶病毒组的典型成员，并且在其生物学的各个方面是最好理解的病毒之一。利用现有的重组RNA病毒和转基因宿主的构建技术，我们提出利用病毒RNA扩增在植物中合成确定的产物。在第二阶段，我们将确定附加体序列的必要和最佳配置，以实现在整个植物中控制和生物包含的高水平基因表达的目标。 植物中最高表达的基因被发现编码在植物RNA病毒基因组中。为了开发作为基因载体的植物病毒，我们将设计保留产生感染性重组体的能力的构建体，因此，将不再限制它们作为基因载体的用途。因此，在商业基础上的复杂分子的生产将大大优化。 ***