Combinatorial Interactions among Eukaryotic Transcription Factors
真核转录因子之间的组合相互作用
基本信息
- 批准号:9404721
- 负责人:
- 金额:$ 30万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Continuing Grant
- 财政年份:1994
- 资助国家:美国
- 起止时间:1994-09-01 至 1998-08-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Abstract 9404721 Promoters in higher eukaryotes tend to have complex structures. In Saccharomyces cerevisiae the upstream activating sequence (UAS) elements of glycolytic enzyme genes closely resemble enhancers and proximal promoter elements of higher eukaryotes in that they are composed of multiple binding sites for several different transcription factors. Each protein binding site by itself displays little UAS activity, but together they form powerful UAS elements. The transcription factors believed to assemble at the UAS elements of glycolytic genes include Abflp, Reb1p, Rap1p, Gcr1p, Gcr2p, and Ga111p. The individual roles of the proteins and how they interact to form the strongest promoters in yeast are not known. The goal of this study is to provide information as to the precise roles played by several of these transcription factors. Specifically: i) The hypothesis that binding of Abflp and Reb1p functions to clear nucleosomes from adjacent binding sites, thereby allowing other proteins access to their binding sites is tested. ii) The importance of spatial relationships between Rap1p- and Gcr1p- binding sites in the UAS elements of glycolytic genes is investigated. iii) Yeast strains with mutations in gcr2 and gal11 are utilized to assess the roles of the co-factors Gcr2p and Gal11p in displacing nucleosomes from the TATA-box. iv) Environmental factors which may influence the ability of transcription factors to bind at glycolytic gene promoters are investigated. These experiments will provide important new information as to how the individual activator proteins interact in a combinatorial fashion at eukaryotic promoters to mediate high-level expression. *** One of the major control points of gene expression is the regulation of transcription. An enzyme known as RNA polymerase is responsible for converting the genetic information from DNA to RNA. Promoters are regions in the DNA which are required to bring RNA polymerase to genes so they can be t ranscribed. They are made of sequences known as the TATA-box, proximal promoters, and enhancers or upstream activating sequences (UAS). Proximal promoters and enhancers or UAS elements are sites in the DNA to which proteins bind. Proteins that bind at these sequences activate transcription by influencing events that occur at the TATA-box. The focus of this study is to elucidate the precise roles played by the proteins that assemble at the UAS elements of glycolytic enzyme genes in the yeast, Saccharomyces cervisiae. In yeast, genes encoding glycolytic enzymes are the most highly expressed. A number of abundant, multifunctional proteins are required for full expression of yeast glycolytic genes. While combinatorial interactions between these proteins at the UAS elements of glycolytic genes has been well documented, the mechanism(s) governing their interactions have yet to be established. The aims of this study are: i) To test a sequential binding hypothesis. ii) To determine the importance of the spatial relationships. iii) To investigate the role of cofactors which are believed to influence events at the TATA-box. iv) To investigate environmental factors which may influence the ability of transcription factors to bind at glycolytic gene UAS elements, thus regulating expression. %%%
摘要高等真核生物中的9404721启动子往往具有复杂的结构。在酿酒酵母中,糖酵解酶基因的上游激活序列(UAS)元件与高等真核生物的增强子和近端启动子元件非常相似,因为它们由多个不同转录因子的结合位点组成。每个蛋白质结合位点本身显示的UAS活性很低,但它们共同形成了强大的UAS元件。据信在糖酵解基因的UAS元件上组装的转录因子包括Abflp、Reb1p、Rap1p、Gcr1p、Gcr2p和Ga111p。这些蛋白质的个体作用以及它们如何相互作用形成酵母中最强的启动子尚不清楚。这项研究的目的是提供有关这些转录因子中的几个所扮演的确切角色的信息。具体地说:i)测试了Abflp和Reb1p结合的假设,即Abflp和Reb1p的结合功能是从邻近的结合部位清除核小体,从而允许其他蛋白质访问它们的结合部位。Ii)研究了糖酵解基因UAS元件中Rap1p和Gcr1p结合位点之间的空间关系的重要性。Iii)利用具有gcr2和Gal11突变的酵母菌株来评估辅助因子Gcr2p和Gal11p在取代TATA盒中的核小体中的作用。Iv)研究了可能影响转录因子与糖酵解基因启动子结合能力的环境因素。这些实验将为单个激活蛋白如何在真核启动子上以组合方式相互作用以介导高水平表达提供重要的新信息。*基因表达的主要控制点之一是转录调控。一种被称为RNA聚合酶的酶负责将遗传信息从DNA转化为RNA。启动子是DNA中的区域,需要将RNA聚合酶带到基因上,以便基因能够被传递。它们由称为TATA-box、近端启动子和增强子或上游激活序列(UA)的序列组成。近端启动子和增强子或UAS元件是DNA中蛋白质结合的位置。结合在这些序列上的蛋白质通过影响发生在TATA盒上的事件来激活转录。这项研究的重点是阐明在酵母中糖酵解酶基因的UAS元件处组装的蛋白质所起的确切作用。在酵母中,编码糖酵解酶的基因表达最高。酵母糖酵解基因的充分表达需要大量的多功能蛋白质。虽然这些蛋白质之间在糖酵解基因的UAS元件上的组合相互作用已经被很好地记录下来,但控制它们相互作用的机制(S)还没有建立。本研究的目的是:i)检验顺序约束假说。二)确定空间关系的重要性。Iii)调查被认为影响塔塔-BOX事件的辅助因素的作用。Iv)研究环境因素可能影响转录因子与糖酵解基因UAS元件结合的能力,从而调节表达。%%%
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Henry Baker其他文献
Henry Baker的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Henry Baker', 18)}}的其他基金
SBIR Phase II: Epipolar-Plane Imaging for Robot 3D Vision
SBIR 第二阶段:机器人 3D 视觉的极面成像
- 批准号:
2242216 - 财政年份:2023
- 资助金额:
$ 30万 - 项目类别:
Cooperative Agreement
SBIR Phase I: A Passive Alternative to LiDAR for Automotive 3D Ranging
SBIR 第一阶段:用于汽车 3D 测距的激光雷达的无源替代方案
- 批准号:
2015152 - 财政年份:2020
- 资助金额:
$ 30万 - 项目类别:
Standard Grant
SBIR Phase I: Fingertip Ranging with Micro Light-Field Cameras
SBIR 第一阶段:使用微光场相机进行指尖测距
- 批准号:
1648388 - 财政年份:2016
- 资助金额:
$ 30万 - 项目类别:
Standard Grant
Combinatorial Interactions Among Eukaryotic Transcriptional Factors
真核转录因子之间的组合相互作用
- 批准号:
9816990 - 财政年份:1999
- 资助金额:
$ 30万 - 项目类别:
Continuing Grant
An Efficient Linear Language for Parallel Symbolic Processing
一种用于并行符号处理的高效线性语言
- 批准号:
9261682 - 财政年份:1993
- 资助金额:
$ 30万 - 项目类别:
Standard Grant
相似海外基金
DISES: Social-ecological drivers and consequences of human-carnivore interactions within and among American cities
疾病:美国城市内部和之间人类与肉食动物相互作用的社会生态驱动因素和后果
- 批准号:
2307324 - 财政年份:2023
- 资助金额:
$ 30万 - 项目类别:
Standard Grant
The Clinical History of Rectal and Urethral STIs among MSM: characterizing microbiome host immune interactions for diagnostic and vaccine advances
MSM 中直肠和尿道 STI 的临床史:表征微生物组宿主免疫相互作用以促进诊断和疫苗进展
- 批准号:
10703680 - 财政年份:2023
- 资助金额:
$ 30万 - 项目类别:
Genetic interactions among targets of master regulator genes as drivers of complex behavior in Drosophila intestinal stem cells
主调节基因靶标之间的遗传相互作用作为果蝇肠道干细胞复杂行为的驱动因素
- 批准号:
10629992 - 财政年份:2023
- 资助金额:
$ 30万 - 项目类别:
Dynamic dyadic parent-child interactions among CI-using children
使用 CI 的儿童之间动态的二元亲子互动
- 批准号:
10740456 - 财政年份:2023
- 资助金额:
$ 30万 - 项目类别:
Understanding the dynamics of animal communities and the maintenance of biodiversity in interactions among spring-fed and runoff streams
了解泉水和径流相互作用中动物群落的动态和生物多样性的维持
- 批准号:
23H02241 - 财政年份:2023
- 资助金额:
$ 30万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Oligopolists' market power in labor and product markets and the interactions among the markets
寡头在劳动力和产品市场的市场力量以及市场之间的相互作用
- 批准号:
23H00818 - 财政年份:2023
- 资助金额:
$ 30万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
BIORETS Biological pathways to adaptability, interactions among the genome, epigenome and environment.
BIORETS 适应性、基因组、表观基因组和环境之间相互作用的生物途径。
- 批准号:
2147083 - 财政年份:2022
- 资助金额:
$ 30万 - 项目类别:
Standard Grant
Computer model of the interactions among members of an inter-professional/interdisciplinary team
跨专业/跨学科团队成员之间互动的计算机模型
- 批准号:
RGPIN-2018-03718 - 财政年份:2022
- 资助金额:
$ 30万 - 项目类别:
Discovery Grants Program - Individual
A Global Map of Interactions Among Human Cell Surface Proteins and Secreted Ligands
人类细胞表面蛋白和分泌配体之间相互作用的全局图
- 批准号:
10710033 - 财政年份:2022
- 资助金额:
$ 30万 - 项目类别:
2023 Glial Biology: Functional Interactions Among Glia and Neurons Gordon Research Conference and Gordon Research Seminar
2023年胶质细胞生物学:胶质细胞和神经元之间的功能相互作用戈登研究会议和戈登研究研讨会
- 批准号:
10609354 - 财政年份:2022
- 资助金额:
$ 30万 - 项目类别:














{{item.name}}会员




