Genetic Analysis of hnRNP Protein Function in Saccharomyces cerevisiae

酿酒酵母hnRNP蛋白功能的遗传分析

• 批准号: 9406969
• 负责人: Sally Amero
• 金额: $ 4.95万
• 依托单位: Loyola University of Chicago
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-06-01 至 1997-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9406969&HistoricalAwards=false
• 关键词: Genetic; Analysis; hnRNP; Protein; Function

9406969 Amero The project is directed towards understanding the mechanism of action of a unique transcription factor, PEP, isolated from Drosophila. The protein is associated with RNA in the nucleus and therefore termed an hnRNP, heterogeneous nuclear ribonucleoprotein. Biochemical characterization of the protein suggests that it may play a role in activating transcription while associated with RNA. This is a very unusual function for an hnRNP and will be investigated more thoroughly. Genetic studies and in vivo analysis of PEP function are difficult in Drosophila. The award will be used to identify and characterize a comparable gene in Saccharomyces cerevisiae. A colleague, Dr. Johns Lopes, has agreed to assist the PI in learning the appropriate yeast manipulations. %%% The project deals with a very unusual protein isolated from fruit flies. The protein is found tightly bound to RNA but seems to also affect the expression of information from DNA. The preliminary characterization of the protein has been carried as far as is reasonably possible using the insect system. To more completely understand the mechanism of action of the protein, the yeast homologue will be characterized and the corresponding gene cloned and mutated. Studies of mutant forms of the protein will greatly facilitate the identification of protein affects gene expression in living cells. ***

小行星9406969 该项目旨在了解从果蝇中分离出的独特转录因子PEP的作用机制。 该蛋白质与细胞核中的RNA结合，因此被称为hnRNP，异质核核糖核蛋白。 该蛋白的生化特征表明，它可能在激活转录中发挥作用，同时与RNA相关。 这对于hnRNP来说是一个非常不寻常的函数，将进行更彻底的研究。 PEP功能的遗传学研究和体内分析在果蝇中是困难的。 该奖项将用于确定和表征酿酒酵母中的可比基因。 一位同事，约翰洛佩斯博士，已同意协助PI学习适当的酵母操作。 该项目涉及一种从果蝇中分离出来的非常不寻常的蛋白质。 这种蛋白质被发现与RNA紧密结合，但似乎也影响DNA信息的表达。 蛋白质的初步表征已经尽可能合理地使用昆虫系统进行。 为了更全面地了解蛋白质的作用机制，将对酵母同源物进行表征，并克隆和突变相应的基因。 对蛋白质突变形式的研究将极大地促进鉴定影响活细胞中基因表达的蛋白质。 ***