A Novel Cell Line and Improved Plant Cell Culture Strategies for the Production of Ribosome Inactivating Proteins

9407177麦当劳这个项目是推进和扩大我们在植物细胞生物技术，核糖体失活蛋白（RIPs）和植物细胞生物过程工程领域的知识。 本项目有三个具体目标，包括：（1）植物病原体发根农杆菌转化植物细胞培养物的表征;（2）悬浮培养中细胞生长和RIP产量的定量; 和（3）从细胞培养物中获得的蛋白质的纯化和表征。 RIPs是天然存在的植物蛋白质，被认为在植物界中普遍存在，并且目前因其抗病毒、抗肿瘤和堕胎特性而受到相当大的关注。 它们还被考虑用于免疫毒素制备的潜在用途以及赋予植物病原体抗性的能力。 ***

9407177 McDonald This project is to advance and broaden our knowledge in the areas of plant cell biotechnology, ribosome inactivating proteins (RIPs), and plant cell bioprocess engineering. This project has three specific goals, which include: (1) characterization of the transformation of plant cell cultures by the plant pathogen Agrobacterium rhizogenes; (2) quantification of cell growth and RIP production in suspension culture; and (3) purification and characterization of proteins obtained from the cell cultures. RIPs are naturally occurring plant proteins thought to be ubiquitous in the plant kingdom, and are currently receiving considerable attention for their antiviral, antitumor and abortifacient properties. They are also being considered for their potential use in immunotoxin preparation and for their ability to confer resistance to plant pathogens. ***