POWRE: A Plant Cell Suspension/Plant Viral System for Expression of Heterologous Proteins

9870453麦当劳麦当劳博士希望使用POWRE奖发起一个新的研究领域，这将有助于她建立一个独立的研究活动。 这个新的领域涉及到用病毒将基因转染到植物细胞悬浮培养物中。 烟草花叶病毒将用于感染烟草、番茄和其他植物细胞。 将使用绿色荧光蛋白标记成功转染。 初始实验将在试管和摇瓶中进行，如果成功，将扩大到5 L生物反应器。 麦克唐纳博士将开发关于植物细胞培养和植物病毒基因转移的新课程材料。 将为高级化学工程设计课程开发一个关于从植物和植物细胞中生产和分离蛋白质的案例研究。 ***

9870453 McDonald Dr. McDonald wishes to use a POWRE award to initiate a new area of research that will aid her in establishing an independent research activity. The new area involves viral transfection of genes to plant cell suspension cultures. Tobacco mosaic virus will be used to infect tobacco, tomato, and other plant cells. Green fluorescent protein will be used to mark successful transfection. The initial experiments will be run in test tubes and shake flasks and will be scalled-up to a 5L bioreactor if successful. Dr. McDonald will develop new course material on plant cell culture and plant virus transfer of genes. A case study on protein production and isolation from plants and plant cells will be developed for a senior chemical engineering design course. ***