Proteins as Copolymers in Hydrophobic Polymers

疏水聚合物中的蛋白质作为共聚物

• 批准号: 9408438
• 负责人: Alan Russell
• 金额: $ 16.12万
• 依托单位: University of Pittsburgh
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-12-01 至 1997-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9408438&HistoricalAwards=false
• 关键词: Proteins; Copolymers; Hydrophobic; Polymers

9408438 Russell This project is on research to study the behavior of enzymes in organic media by attachment of an enzyme to an organic polymer. In particular, an enzyme will be fixed to a hydrophobic matrix of polyethylene glycol (PEG) chains. This would allow the enzyme to dissolve in an organic solvent while maintaining activity. The enzyme would be attached to a polymer by: (1) direct functionalization of the enzyme with an acrylate-terminal PEG to produce an enzyme in an organic solvent, followed by (2) solution copolymerization of the enzyme macromonomer with a vinyl monomer. Studies of the enzyme stability, activity and specificity in this environment, as well as studies on the effects of polymer macrostructure on the overall kinetics of the enzyme-catalyzed reaction will be performed. Also, a study of protein activity as effected by polymer porosity, enzyme loading, the extent of enzyme modification prior to immobilization, polymer hydrophobicity, other physical properties, and mass transfer will be performed. The particular enzyme to be used in this research is organophosphorous hydrolase, and enzyme which can degrade certain insecticides such as parathion, and certain types of nerve gases. This research could have environmental impact through the biodegradation of highly toxic compounds. ***

9408438 Russell该项目是通过将酶附着到有机聚合物上来研究酶在有机介质中的行为。 特别地，酶将被固定到聚乙二醇（PEG）链的疏水基质上。 这将允许酶溶解在有机溶剂中，同时保持活性。 酶将通过以下方式连接到聚合物上：（1）用丙烯酸酯末端PEG直接官能化酶以在有机溶剂中产生酶，随后（2）酶大分子单体与乙烯基单体的溶液共聚。 将进行酶在这种环境中的稳定性、活性和特异性的研究，以及聚合物宏观结构对酶催化反应的整体动力学的影响的研究。 此外，将进行蛋白质活性受聚合物孔隙率、酶负载、固定化前酶修饰程度、聚合物疏水性、其他物理性质和传质影响的研究。 在这项研究中使用的特定酶是有机磷水解酶，以及可以降解某些杀虫剂如敌百虫和某些类型的神经毒气的酶。 这项研究可能通过剧毒化合物的生物降解对环境产生影响。 ***