Expression & Function of the Tomato B-Subunit During Growth and Development

表达

• 批准号: 9408826
• 负责人: Dean DellaPenna
• 金额: $ 18.16万
• 依托单位: University of Arizona
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-07-15 至 1996-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9408826&HistoricalAwards=false
• 关键词: Expression; & Function; Tomato; Subunit

9408826 DellaPenna A long-term goal of this research is understanding the functional significance of developmentally regulated, polyuronide modifications in plants. As part of prior NSF funding, the Beta- subunit of polygalacturonase (PG1) was purified; cDNAs encoding the protein were isolated and characterized. Beta-subunit mRNA accumulates to high levels in developing fruit and is to a large degree temporally separated from catalytic PG2 expression. Homologous sequences have been identified in a wide variety of plant species suggesting a broad functional role for the protein in plants. The current working theory is that the Beta-subunit defines a new class of bifunctional plant cell wall proteins that interact both with structural components of the wall and catalytic proteins, to localize and/or regulate metabolic activities within the cell wall. This hypothesis can now be tested in transgenic tomoto fruit in which Beta-subunit expression is inhibited by antisense RNA. The proposed research will further address long- standing questions of how pectin-degrading enzymes are targeted and regulated in plants and what the functional significance of pectin degradation is in various tissues and cell types. ***

9408826 DellaPenna本研究的长期目标是了解植物中发育调节的多糖苷酸修饰的功能意义。 作为先前NSF资助的一部分，纯化了多聚半乳糖醛酸酶（PG 1）的β亚基;分离并表征了编码该蛋白的cDNA。 β-亚基mRNA在发育中的果实中积累到高水平，并且在很大程度上与催化PG 2表达在时间上分离。 同源序列已在多种植物物种中鉴定，表明该蛋白在植物中具有广泛的功能作用。 目前的工作理论是，β-亚基定义了一类新的双功能植物细胞壁蛋白，其与壁的结构组分和催化蛋白相互作用，以定位和/或调节细胞壁内的代谢活动。 这一假设现在可以在转基因tomoto果实中进行测试，其中β亚基表达被反义RNA抑制。 拟议的研究将进一步解决长期存在的问题，即果胶降解酶如何在植物中被靶向和调节，以及果胶降解在各种组织和细胞类型中的功能意义。 ***