Acquisition of an Advanced Flow Cytometer

购置先进的流式细胞仪

• 批准号: 9413418
• 负责人: Donald Weeks
• 金额: $ 25.62万
• 依托单位: University of Nebraska-Lincoln
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-09-01 至 1996-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9413418&HistoricalAwards=false
• 关键词: Acquisition; Advanced; Flow; Cytometer

With this proposal, we request funding for the purchase of a multi-purpose state-of-the-art flow cytometer / cell sorter (Becton Dickinson FACS VantageTM Cell Sorter system or Coulter EPICS Elite ESP system) to update and expand our current multi-user Flow Cytometry Core Research Facility of the University of Nebraska-Lincoln (UNL) Center for Biotechnology. The new equipment will replace our fourteen year-old Coulter EPICS V system which now requires frequent repairs. This instrument has only a short life span remaining. The present flow cytometer is also inadequate for the needs of several investigators on the UNL campus who wish to perform bivariate chromosome analysis, multicolor fluorescence analysis, and state-of-the-art sorting of chromosomes and cells in their research endeavors. As a multipurpose facility, we are requesting accessories for a new flow cytometer which will permit: a user friendly operating system; multicolor fluorescence analysis and at least two laser capability; cell sorting in less time; computerized drop-delay setup; large-particle analysis and sorting; sorting of 1-100,000 cells into microtitier trays, onto microscope slides or filters. Importantly, the new flow cytometer system we have chosen is designed to accommodate future upgrades and applications. Even with the limitations of the present instrumentation, projects in over 25 research laboratories have benefited from the services provided by the Flow Cytometry Core Research Facility. Acquisition of a state-of-the-art instrument will better service current users and most certainly will attract additional users whose needs cannot be met with the instrument presently in use. Examples of specific research activities that would result from the availability the new instrument include: 1) Univariate and bivariate flow karyotyping and sorting of chromosomes from important plant species for the purpose of construction of chromosome-specific DNA libraries. Work is already in progress with tomato and corn chromosomes. We will actively seek collaboration from genome researchers throughout the world who want sorted chromosomes from other important plants. Our goal is to make our Flow Cytometry Core Research Facility an internationally recognized center for plant genome analysis and chromosome sorting research. 2) Flow cytometric analysis of nuclei of animals and plants (both crop plants and native plants), and cell lines from animals and plants for the determination of ploidy level and/or estimation of nuclear DNA content. 3) Immunofluorescence analysis (1-4 color) and sorting of blood cell subsets and cell lines for the expression of surface antigens, receptors or internal substances.

通过此提案，我们请求资金购买多用途最先进的流式细胞仪/细胞分选仪（Becton Dickinson FACS VantageTM 细胞分选仪系统或 Coulter EPICS Elite ESP 系统），以更新和扩展我们内布拉斯加大学林肯分校 (UNL) 生物技术中心当前的多用户流式细胞术核心研究设施。新设备将取代我们已有 14 年历史的 Coulter EPICS V 系统，该系统现在需要频繁维修。该仪器仅剩很短的使用寿命。目前的流式细胞仪也不足以满足 UNL 校园内几位研究人员的需求，他们希望在研究工作中进行双变量染色体分析、多色荧光分析以及最先进的染色体和细胞分选。作为一个多功能设施，我们正在请求新型流式细胞仪的配件，该配件将允许： 用户友好的操作系统；多色荧光分析和至少两种激光能力；在更短的时间内进行细胞分选； 计算机化的下降延迟设置；大颗粒分析和分选； 将 1-100,000 个细胞分选到微量滴定盘、显微镜载玻片或过滤器上。重要的是，我们选择的新流式细胞仪系统旨在适应未来的升级和应用。 尽管现有仪器存在局限性，超过 25 个研究实验室的项目仍受益于流式细胞术核心研究设施提供的服务。购买最先进的仪器将更好地为当前用户提供服务，并且肯定会吸引目前使用的仪器无法满足其需求的其他用户。 新仪器的可用性将导致具体研究活动的例子包括： 1) 单变量和双变量流式核型分析以及对重要植物物种的染色体进行分类，以构建染色体特异性 DNA 文库。 番茄和玉米染色体的研究工作已经在进行中。我们将积极寻求世界各地想要从其他重要植物中分选染色体的基因组研究人员的合作。我们的目标是使我们的流式细胞术核心研究设施成为国际公认的植物基因组分析和染色体分选研究中心。 2) 对动物和植物（农作物和本地植物）以及来自动物和植物的细胞系进行流式细胞术分析，以确定倍性水平和/或估计核DNA含量。 3) 免疫荧光分析（1-4色）和血细胞亚群和细胞系的表面抗原、受体或内部物质表达的分选。