Molecular and Genetic Analyses of the Carbon Concentrating Mechanism of Chlamydomonas reinhardtii

莱茵衣藻碳富集机制的分子和遗传学分析

• 批准号: 9723333
• 负责人: Donald Weeks
• 金额: $ 26万
• 依托单位: University of Nebraska-Lincoln
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-15 至 2001-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9723333&HistoricalAwards=false
• 关键词: Molecular; Genetic; Analyses; Carbon; Concentrating

9723333 Weeks The primary goal of the research is to elucidate components and mechanisms of regulation of the carbon concentration mechanism in this alga. The work originates from the detection of gene CAH3 through genomic complementation of a high CO2-requiring mutant strain. Additional genes are being isolated following this screen. A C02-responsive promoter will be used to construct a gene cassette that allows screening for a selectable marker in another approach to defining mutants in the signal transduction chain leading from CO2 sensing to gene expression. These mutants will be characterized.

9723333周研究的主要目标是阐明该藻类中碳浓缩机制的组成和调节机制。这项工作源于通过对一株高二氧化碳需求突变菌株的基因组互补来检测CAH3基因。在这一筛选之后，更多的基因正在被分离。在另一种定义从二氧化碳感应到基因表达的信号转导链中突变的方法中，将使用对二氧化碳响应的启动子构建基因盒，以筛选可选择的标记。这些突变体将被描述为。