Acquisition of Instrumentation for Enzyme Assembly Research

这份提案概述了购买仪器的要求，以支持学生/教师在酶组装的分子分析方面的研究。该项目的目标是建立一个分子生物学实验室，为酵母液泡膜质子转运ATPase(V-ATPase)的组装和功能研究提供支持。该仪器要求包括1)桌面超速离心机，2)自动低压层析系统，3)DNA测序设备，4)成像密度计，5)核酸杂交设备，6)细胞电穿孔仪，7)酵母培养设备。每个仪器都支持一种特定的实验方法，该方法是本提案中描述的研究计划的组成部分。

This proposal outlines a request for instrumentation acquisition to support student/faculty research in molecular analysis of enzyme assembly. The goal of this project is to build a molecular biology laboratory that will support research on the assembly and function of the proton- translocating ATPase in the yeast vacuolar membrane (V- ATPase). This instrumentation request includes 1) a tabletop ultracentrifuge, 2) an automated low pressure chromatography system, 3) DNA sequencing equipment, 4) an imaging densitometer, 5) nucleic acid hybridization equipment, 6) a cell electroporator, and 7) yeast culturing equipment. Each instrument supports a specific experimental approach that is an integral part of the research program described in this proposal.