Acquisition of Instrumentation for Enzyme Assembly Research

该提案概述了仪器采购的要求，以支持学生/教师在酶组装的分子分析研究。 本项目的目标是建立一个分子生物学实验室，以支持对质子转运ATP酶在酵母液泡膜中的组装和功能的研究。该仪器要求包括1）台式超浓缩仪，2）自动低压色谱系统，3）DNA测序设备，4）成像密度计，5）核酸杂交设备，6）细胞电穿孔仪，以及7）酵母培养设备。每种仪器都支持一种特定的实验方法，这是本提案中描述的研究计划的一个组成部分。

This proposal outlines a request for instrumentation acquisition to support student/faculty research in molecular analysis of enzyme assembly. The goal of this project is to build a molecular biology laboratory that will support research on the assembly and function of the proton- translocating ATPase in the yeast vacuolar membrane (V- ATPase). This instrumentation request includes 1) a tabletop ultracentrifuge, 2) an automated low pressure chromatography system, 3) DNA sequencing equipment, 4) an imaging densitometer, 5) nucleic acid hybridization equipment, 6) a cell electroporator, and 7) yeast culturing equipment. Each instrument supports a specific experimental approach that is an integral part of the research program described in this proposal.