Acquisition of Instrumentation for Enzyme Assembly Research

购置酶组装研究仪器

• 批准号: 9413764
• 负责人: Olaf Runquist
• 金额: $ 17.93万
• 依托单位: Hamline University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-09-15 至 1996-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9413764&HistoricalAwards=false
• 关键词: Acquisition; Instrumentation; Enzyme; Assembly; Research

This proposal outlines a request for instrumentation acquisition to support student/faculty research in molecular analysis of enzyme assembly. The goal of this project is to build a molecular biology laboratory that will support research on the assembly and function of the proton- translocating ATPase in the yeast vacuolar membrane (V- ATPase). This instrumentation request includes 1) a tabletop ultracentrifuge, 2) an automated low pressure chromatography system, 3) DNA sequencing equipment, 4) an imaging densitometer, 5) nucleic acid hybridization equipment, 6) a cell electroporator, and 7) yeast culturing equipment. Each instrument supports a specific experimental approach that is an integral part of the research program described in this proposal.

该提案概述了仪器采购的要求，以支持学生/教师在酶组装的分子分析研究。 本项目的目标是建立一个分子生物学实验室，以支持对质子转运ATP酶在酵母液泡膜中的组装和功能的研究。该仪器要求包括1）台式超浓缩仪，2）自动低压色谱系统，3）DNA测序设备，4）成像密度计，5）核酸杂交设备，6）细胞电穿孔仪，以及7）酵母培养设备。每种仪器都支持一种特定的实验方法，这是本提案中描述的研究计划的一个组成部分。