Transmembrane Signaling By Receptor Protein Kinases In Plants

植物中受体蛋白激酶的跨膜信号传导

基本信息

  • 批准号:
    9417732
  • 负责人:
  • 金额:
    $ 34.5万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Continuing Grant
  • 财政年份:
    1995
  • 资助国家:
    美国
  • 起止时间:
    1995-04-15 至 1998-09-30
  • 项目状态:
    已结题

项目摘要

MCB-9417732 Walker Differentiation in plants is profoundly affected by environmental factors. The long term goal of our research is to understand how extracellular signals interact with the plant cellular machinery to influence growth and development. Recent evidence strongly suggests that plant cells have plasma membrane receptors with intrinsic protein kinase activity. The plant receptor-like protein kinases (RLK) are structurally related to the animal polypeptide growth factor receptors; however, the plant RLKs all appear to be serine/threonine protein kinases. Diversity among plant RLKs, reflected in their structural and functional properties, has opened up a broad new area of investigation into cellular signaling in plants with far-reaching implications for the mechnaisms by which plant cells perceive and respond to extracellular stimuli. Our specific aims are to characterize the biochemical properties, both in vitro and in vivo, of one receptor-like protein kinase (RLK5) from Arabidopsis thaliana. We have shown that a recombinant fusion protein of this transmembrane protein kinase will autophosphorylate several sites and that autophosphorylation is critical for interaction with cellular targets. One of these targets, KAPP (kinase associated protein phosphatase) contains three domains: an amino-terminal membrane insertion signal, a kinase interaction domain and phosphoprotein phosphatase domain, Interaction of KAPP with RLK5 requires RLK5 phosphorylation. This mechanism of binding is reminiscent of the interaction of src homology 2 containing proteins with activated growth factor receptors in animals. The research proposed here will define the autophosphorylation sites in RLK5 and investigate the functional significance of KAPP binding to RLK5. Additional components for RLK5 mediated intracellular signaling will also be identified. It is anticipated that results from these studies will yield fundamental and new insights into the molecular mech anisms of how plants perceive their environment and how growth and developmental processes are regulated by environmental signals. %%% Differentiation in plants is profoundly affected by environmental factors. The long term goal of our research is to understand how extracellular signals interact with the plant cellular machinery to influence growth and development. Recent evidence strongly suggests that plant cells have plasma membrane receptors with intrinsic protein kinase activity. The plant receptor-like protein kinases (RLK) are structurally related to the animal polypeptide growth factor receptors; however, the plant RLKs all appear to be serine/threonine protein kinases. Diversity among plant RLKs, reflected in their structural and functional properties, has opened up a broad new area of investigation into cellular signaling in plants with far-reaching implications for the mechnaisms by which plant cells perceive and respond to extracellular stimuli. Our specific aims are to characterize the biochemical properties, both in vitro and in vivo, of one receptor-like protein kinase (RLK5) from Arabidopsis thaliana. We have shown that a recombinant fusion protein of this transmembrane protein kinase will autophosphorylate several sites and that autophosphorylation is critical for interaction with cellular targets. The work will now further investigate the nature of the autophosphorylation and how it is important in the signaling sequence of events. It is anticipated that results from these studies will yield fundamental and new insights into the molecular mechanisms of how plants perceive their environment and how growth and developmental processes are regulated by environmental signals. ***
MCB-9417732步行者 植物的分化受到环境因素的深刻影响。 我们研究的长期目标是了解细胞外信号如何与植物细胞机制相互作用以影响生长和发育。 最近的证据有力地表明,植物细胞具有质膜受体与内在的蛋白激酶活性。 植物受体样蛋白激酶(RLK)在结构上与动物多肽生长因子受体相关;然而,植物RLK似乎都是丝氨酸/苏氨酸蛋白激酶。 植物RLKs的多样性,反映在它们的结构和功能特性,开辟了一个广阔的新领域的研究细胞信号在植物中具有深远的意义的mechannaisms,植物细胞感知和响应胞外刺激。 我们的具体目标是表征的生化特性,在体外和体内,一个受体样蛋白激酶(RLK 5)从拟南芥。 我们已经表明,这种跨膜蛋白激酶的重组融合蛋白将自磷酸化几个网站和自磷酸化是至关重要的与细胞靶点的相互作用。 KAPP(kinase associated protein phosphatase,激酶相关蛋白磷酸酶)包含三个结构域:氨基端膜插入信号、激酶相互作用结构域和磷蛋白磷酸酶结构域,KAPP与RLK 5的相互作用需要RLK 5磷酸化。 这种结合机制让人想起动物中src同源2蛋白与活化的生长因子受体的相互作用。 本研究将确定RLK 5的自磷酸化位点,并研究KAPP与RLK 5结合的功能意义。 还将鉴定用于RLK 5介导的细胞内信号传导的其他组分。 预计这些研究的结果将对植物如何感知环境以及环境信号如何调节生长和发育过程的分子机制产生根本性的和新的见解。 植物的分化受到环境因素的深刻影响。 我们研究的长期目标是了解细胞外信号如何与植物细胞机制相互作用以影响生长和发育。 最近的证据有力地表明,植物细胞具有质膜受体与内在的蛋白激酶活性。 植物受体样蛋白激酶(RLK)在结构上与动物多肽生长因子受体相关;然而,植物RLK似乎都是丝氨酸/苏氨酸蛋白激酶。 植物RLKs的多样性,反映在它们的结构和功能特性,开辟了一个广阔的新领域的研究细胞信号在植物中具有深远的意义的mechannaisms,植物细胞感知和响应胞外刺激。 我们的具体目标是表征的生化特性,在体外和体内,一个受体样蛋白激酶(RLK 5)从拟南芥。 我们已经表明,这种跨膜蛋白激酶的重组融合蛋白将自磷酸化几个网站和自磷酸化是至关重要的与细胞靶点的相互作用。 这项工作现在将进一步研究自磷酸化的性质以及它在事件的信号序列中的重要性。 预计这些研究的结果将对植物如何感知其环境以及生长和发育过程如何受环境信号调节的分子机制产生基本和新的见解。 ***

项目成果

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John David其他文献

Interstitial high-dose-rate brachytherapy in the treatment of keloids: Moving toward a volumetric approach
  • DOI:
    10.1016/j.brachy.2020.07.008
  • 发表时间:
    2021-01-01
  • 期刊:
  • 影响因子:
  • 作者:
    Eric M. Anderson;John David;Tiffany Phillips;John Demarco;Robert S. Reznik;Amin J. Mirhadi;Mitchell Kamrava
  • 通讯作者:
    Mitchell Kamrava
How do gardeners define ‘invasive’? Implications for invasion science and environmental policy instruments on invasive species
园丁如何定义“入侵”对入侵科学和环境政策工具对入侵物种的影响?
  • DOI:
    10.1016/j.envsci.2023.103614
  • 发表时间:
    2024
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Tomos Siôn Jones;A. Culham;B. J. Pickles;John David
  • 通讯作者:
    John David
Demonstrations, Grievance, and Civil Conflict
示威、申诉和国内冲突
Annals of Clinical Microbiology and Antimicrobials Activity of Telithromycin and Comparators against Bacterial Pathogens Isolated from 1,336 Patients with Clinically Diagnosed Acute Sinusitis
泰利霉素和对照药物对 1,336 名临床诊断的急性鼻窦炎患者分离的细菌病原体的临床微生物学和抗菌活性年鉴
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
    J. Dohar;R. Cantón;R. Cohen;D. J. Farrell;D. Felmingham;Edu;Cantón;Es;Cohen;Fr;John David;D. F. Farrell
  • 通讯作者:
    D. F. Farrell
Can gardeners identify ‘future invaders’?
园丁能识别“未来的入侵者”吗?
  • DOI:
  • 发表时间:
    2024
  • 期刊:
  • 影响因子:
    5.1
  • 作者:
    Tomos Siôn Jones;A. Culham;B. J. Pickles;John David
  • 通讯作者:
    John David

John David的其他文献

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{{ truncateString('John David', 18)}}的其他基金

Scholarships to Enhance Recruitment and Retention of Underserved and Underrepresented Students in Biology
奖学金旨在加强生物学领域服务不足和代表性不足的学生的招募和保留
  • 批准号:
    0965807
  • 财政年份:
    2010
  • 资助金额:
    $ 34.5万
  • 项目类别:
    Continuing Grant
REU Site: Cellular, Molecular and Genetic Approaches to Biological Problems
REU 网站:解决生物学问题的细胞、分子和遗传学方法
  • 批准号:
    0850689
  • 财政年份:
    2009
  • 资助金额:
    $ 34.5万
  • 项目类别:
    Standard Grant
Scholarships to Enhance Recruitment and Retention of Underserved and Underrepresented Students in Biology
奖学金旨在加强生物学领域服务不足和代表性不足的学生的招募和保留
  • 批准号:
    0630742
  • 财政年份:
    2006
  • 资助金额:
    $ 34.5万
  • 项目类别:
    Standard Grant
REU Site: Cellular, Molecular and Genetic Approaches to Biological Problems
REU 网站:解决生物学问题的细胞、分子和遗传学方法
  • 批准号:
    0243880
  • 财政年份:
    2003
  • 资助金额:
    $ 34.5万
  • 项目类别:
    Continuing Grant
REU Site: Cellular, Molecular and Genetic Approaches to Biological Problems
REU 网站:解决生物学问题的细胞、分子和遗传学方法
  • 批准号:
    9988022
  • 财政年份:
    2000
  • 资助金额:
    $ 34.5万
  • 项目类别:
    Continuing Grant
Cellular, Molecular and Genetic Approaches to Biological Problems - REU Site
解决生物问题的细胞、分子和遗传学方法 - REU 网站
  • 批准号:
    9423891
  • 财政年份:
    1995
  • 资助金额:
    $ 34.5万
  • 项目类别:
    Continuing Grant
REU:Cellular, Molecular and Genetic Approaches to BiologicalProblems
REU:解决生物问题的细胞、分子和遗传学方法
  • 批准号:
    9300294
  • 财政年份:
    1993
  • 资助金额:
    $ 34.5万
  • 项目类别:
    Continuing Grant
Cellular, Molecular and Genetic Approaches in Biological Problems
生物学问题中的细胞、分子和遗传学方法
  • 批准号:
    9200065
  • 财政年份:
    1992
  • 资助金额:
    $ 34.5万
  • 项目类别:
    Standard Grant
REU: Cellular, Molecular and Genetic Approaches to to Biological Problems
REU:解决生物学问题的细胞、分子和遗传学方法
  • 批准号:
    9100571
  • 财政年份:
    1991
  • 资助金额:
    $ 34.5万
  • 项目类别:
    Standard Grant
Acquisition of an Ultracentrifuge
购买超速离心机
  • 批准号:
    8413616
  • 财政年份:
    1985
  • 资助金额:
    $ 34.5万
  • 项目类别:
    Standard Grant

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Mechanisms of Integrin Receptor Transmembrane Signaling
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Mechanisms of Integrin Receptor Transmembrane Signaling
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